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Replacing Arginine 33 for Alanine in the Hemophore HasA from Pseudomonas aeruginosa Causes Closure of the H32 Loop in the Apo-Protein
(ACS Publications, 2016-05)Previous characterization of hemophores from Serratia marcescens (HasAs), Pseudomonas aeruginosa (HasAp) and Yersinia pestis (HasAyp) showed that hemin binds between two loops, where it is axially coordinated by H32 and ... -
Microfluidic Exosome Analysis toward Liquid Biopsy for Cancer
(Sage, 2016-08)Assessment of a tumor’s molecular makeup using biofluid samples, known as liquid biopsy, is a prominent research topic in precision medicine for cancer, due to its noninvasive property allowing repeat sampling for monitoring ... -
GlycoPep MassList: Software to Generate Massive Inclusion Lists for Glycopeptide Analyses
(Springer Verlag, 2017-01)Protein glycosylation drives many biological processes and serves as markers for disease; therefore, the development of tools to study glycosylation is an essential and growing area of research. Mass spectrometry can be ... -
Spectroscopic and Computational Investigation of Low-Spin MnIII Bis(scorpionate) Complexes
(Wiley, 2016-06)Six-coordinate MnIII complexes are typically high-spin (S = 2), however, the scorpionate ligand, both in its traditional, hydridotris(pyrazolyl)borate form, Tp– and Tp*– (the latter with 3,5-dimethylpyrazole substituents) ... -
Metal complexes with varying intramolecular hydrogen bonding networks
(Elsevier, 2013-03-01)Alfred Werner described the attributes of the primary and secondary coordination spheres in his development of coordination chemistry. To examine the effects of the secondary coordination sphere on coordination chemistry, ... -
2-Diazo-1-(4-hydroxyphenyl)ethanone: A Versatile Photochemical and Synthetic Reagent
(Royal Society of Chemistry, 2014-02)α-Diazo arylketones are well-known substrates for Wolff rearrangement to phenylacetic acids through a ketene intermediate by either thermal or photochemical activation. Likewise, α-substituted p-hydroxyphenacyl (pHP) esters ... -
The permeation of dynorphin A 1–6 across the blood brain barrier and its effect on bovine brain microvessel endothelial cell monolayer permeability
(Elsevier, 2012-12)Dynorphin A 1–17 (Dyn A 1–17) is an endogenous neuropeptide known to act at the kappa opioid receptor; it has been implicated in a number of neurological disorders, including neuropathic pain, stress, depression, and ... -
Enthalpy vs Entropy Driven Complexation of Homoallylic Alcohols by Rh(I) Complexes
(American Chemical Society, 2011-10-25)The thermodynamics of binding between several homoallylic alcohols and simple olefinic Rh(I) compounds was examined with 1H NMR spectroscopy and ITC. 1H NMR titrations revealed moderate binding of these alcohols with ... -
LipidomeDB Data Calculation Environment: Online Processing of Direct-Infusion Mass Spectral Data for Lipid Profiles
(Springer Verlag, 2011-09)LipidomeDB Data Calculation Environment (DCE) is a web application to quantify complex lipids by processing data acquired after direct infusion of a lipid-containing biological extract, to which a cocktail of internal ... -
Fuming Method for Micropatterning Structures on Langmuir- Blodgett Films
(American Chemical Society, 2009-05-05)Lipid monolayers of L-α-dipalmitoylphosphatidylcholine (DPPC) are used to pattern substrates using the Langmuir-Blodgett (LB) technique. Lipid monolayers are deposited onto a freshly cleaved mica surface or glass capillary ... -
Isotope Effects and Temperature Dependences in the Action of the Glucose Dehydrogenase of the Mesophilic Bacterium Bacillus megaterium
(Wiley, 2013-12-01)The glucose dehydrogenase of the mesophilic bacterium Bacillus megaterium (optimal growth around 35 °C) exhibits non-linear Eyring temperature dependences from 25 to 55 °C in its catalysis of the oxidation by hydride-transfer ... -
Partial cooperative unfolding in proteins as observed by hydrogen exchange mass spectrometry
(Taylor & Francis, 2013-01-01)Many proteins do not exist in a single rigid conformation. Protein motions, or dynamics, exist and in many cases are important for protein function. The analysis of protein dynamics relies on biophysical techniques that ... -
Methods development for Analysis of Partially Deglycosylated Proteins and Application to an HIV Envelope Protein Vaccine Candidate
(Elsevier, 2011-08)The work presented herein describes the first comprehensive analysis of a partially deglycosylated HIV vaccine candidate envelope protein (Env). The Env, JRFL gp140 ΔCF, with 27 potential glycosylation sites, was partially ... -
MAPping the Chiral Inversion and Structural Transformation of a Metal-Tripeptide Complex having Ni-SOD Activity
(American Chemical Society, 2011-03-21)The metal abstraction peptide (MAP) tag is a tripeptide sequence capable of abstracting a metal ion from a chelator and binding it with extremely high affinity at neutral pH. Initial studies on the nickel-bound form of the ... -
Embedding the Ni-SOD mimetic Ni-NCC within a polypeptide sequence alters specificity of the reaction pathway
(American Chemical Society, 2013-01-07)The unique metal abstracting peptide (MAP) asparagine-cysteine-cysteine (NCC) binds nickel in a square planar 2N:2S geometry and acts as a mimic of the enzyme nickel superoxide dismutase (Ni-SOD). The Ni-NCC tripeptide ... -
Controlling the Chiral Inversion Reaction of the Metallopeptide Ni-Asparagine-Cysteine-Cysteine with Dioxygen
(American Chemical Society, 2012-09-17)Synthetically generated metallopeptides have the potential to serve a variety of roles in biotechnology applications, but the use of such systems is often hampered by the inability to control secondary reactions. We have ... -
Synthesis of a Library of 1,5,2-Dithiazepine 1,1-Dioxides. Part 2: Routes to Bicyclic Sultams
(Elsevier, 2012-12-31)The synthesis of a library of bicyclic sultams incorporating the 1,5,2-dithiazepine 1,1-dioxide moiety is reported. Following scaffold synthesis via a one-pot sulfonylation/intramolecular thia-Michael protocol, several ... -
Synthesis of a Library of 1,5,2-Dithiazepine 1,1-Dioxides. Part 1: A One-Pot Sulfonylation/Thia-Michael Protocol
(Elsevier, 2012-12-31)A novel one-pot sulfonylation/intramolecular thia-Michael protocol is reported for the synthesis of 1,5,2-dithiazepine 1,1-dioxides. Sulfonylation between cysteine ethyl ester/cysteamine and 2-chloroethanesulfonyl chloride, ... -
A novel tripeptide model of nickel superoxide dismutase
(American Chemical Society, 2010-01-18)Nickel superoxide dismutase (Ni-SOD) catalyzes the disproportionation of superoxide to molecular oxygen and hydrogen peroxide, but the overall reaction mechanism has yet to be determined. Peptide-based models of the 2N:2S ... -
Fluorescence quenching studies of structure and dynamics in calmodulin-eNOS complexes
(Wiley, 2015-05-08)Activation of endothelial nitric oxide synthase (eNOS) by calmodulin (CaM) facilitates formation of a sequence of conformational states that is not well understood. Fluorescence decays of fluorescently labeled CaM bound ...
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