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    Illuminating the diversity of aromantic polyketide synthases in Aspergillus nidulans

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    Ahuja_2012.pdf (1.920Mb)
    Issue Date
    2012-05-16
    Author
    Ahuja, Manmeet
    Chiang, Yi-Ming
    Chang, Shu-Lin
    Praseuth, Mike B.
    Entwistle, Ruth A.
    Sanchez, James F.
    Lo, Hsien-Chun
    Yeh, Hsu-Hua
    Oakley, Berl R.
    Wang, Clay C. C.
    Publisher
    American Chemical Society
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
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    Abstract
    Genome sequencing has revealed that fungi have the ability to synthesize many more natural products (NPs) than are currently known, but methods for obtaining suitable expression of NPs have been inadequate. We have developed a successful strategy that bypasses normal regulatory mechanisms. By efficient gene targeting, we have replaced, en masse, the promoters of non-reducing polyketide synthase (NR-PKS) genes, key genes in NP biosynthetic pathways and other genes necessary for NR-PKS product formation or release. This has allowed us to determine the products of eight NR-PKSs of A. nidulans, including seven novel compounds, as well as the NR-PKS genes required for the synthesis of the toxins, alternariol (8) and cichorine (19).
    Description
    This document is the Accepted Manuscript version of a Published Work that appeared in final form in the Journal of the American Chemical Society, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/ja3016395.
    URI
    http://hdl.handle.net/1808/24379
    DOI
    https://doi.org/10.1021/ja3016395
    Collections
    • Molecular Biosciences Scholarly Works [537]
    Citation
    Ahuja, M., Chiang, Y.-M., Chang, S.-L., Praseuth, M. B., Entwistle, R., Sanchez, J. F., … Wang, C. C. C. (2012). Illuminating the diversity of aromatic polyketide synthases in Aspergillus nidulans. Journal of the American Chemical Society, 134(19), 8212–8221. http://doi.org/10.1021/ja3016395

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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