A Peptide from the Beta-strand Region of CD2 Protein that Inhibits Cell Adhesion and Suppresses Arthritis in a Mouse Model
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Issue Date
2010-09-01Author
Satyanarayanajois, Seetharama D.
Büyüktimkin, Barlas
Gokhale, Ameya
Ronald, Sharon
Siahaan, Teruna J.
Latendresse, John R.
Publisher
Wiley
Type
Article
Article Version
Scholarly/refereed, author accepted manuscript
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Show full item recordAbstract
Cell adhesion molecules play a central role at every step of the immune response. The function of leukocytes can be regulated by modulating adhesion interactions between cell adhesion molecules to develop therapeutic agents against autoimmune diseases. Among the different cell adhesion molecules that participate in the immunological response, CD2 and its ligand CD58 (LFA-3) are two of the best-characterized adhesion molecules mediating the immune response. To modulate the cell adhesion interaction, peptides were designed from the discontinuous epitopes of the β-strand region of CD2 protein. The two strands were linked by a peptide bond. β-Strands in the peptides were nucleated by inserting a β-sheet-inducing Pro-Gly sequence with key amino acid sequences from CD2 protein that binds to CD58. Using a fluorescence assay, peptides that exhibited potential inhibitory activity in cell adhesion were evaluated for their ability to bind to CD58 protein. A model for peptide binding to CD58 protein was proposed based on docking studies. Administration of one of the peptides, P3 in collagen-induced arthritis (CIA) in the mouse model, indicated that peptide P3 was able to suppress rheumatoid arthritis in mice.
Description
This is the peer reviewed version of the following article: Satyanarayanajois, S. D., Büyüktimkin, B., Gokhale, A., Ronald, S., Siahaan, T. J. and Latendresse, J. R. (2010), A Peptide from the Beta-strand Region of CD2 Protein that Inhibits Cell Adhesion and Suppresses Arthritis in a Mouse Model. Chemical Biology & Drug Design, 76: 234–244. doi:10.1111/j.1747-0285.2010.01001.x, which has been published in final form at http://doi.org/10.1111/j.1747-0285.2010.01001.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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Citation
Satyanarayanajois, S. D., Büyüktimkin, B., Gokhale, A., Ronald, S., Siahaan, T. J. and Latendresse, J. R. (2010), A Peptide from the Beta-strand Region of CD2 Protein that Inhibits Cell Adhesion and Suppresses Arthritis in a Mouse Model. Chemical Biology & Drug Design, 76: 234–244. doi:10.1111/j.1747-0285.2010.01001.x
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