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  • Development of spirulina for the manufacture and oral delivery of protein therapeutics 

    Jester, Benjamin W.; Zhao, Hui; Gewe, Mesfin; Adame, Thomas; Perruzza, Lisa; Bolick, David T.; Agosti, Jan; Khuong, Nhi; Kuestner, Rolf; Gamble, Caitlin; Cruickshank, Kendra; Ferrara, Jeremy; Lim, Rachelle; Paddock, Troy; Brady, Colin; Ertel, Stacey; Zhang, Miaohua; Pollock, Alex; Lee, Jamie; Xiong, Jian; Tasch, Michael; Saveria, Tracy; Doughty, David; Marshall, Jacob; Carrieri, Damian; Goetsch, Lauren; Dang, Jason; Sanjaya, Nathaniel; Fletcher, David; Martinez, Anissa; Kadis, Bryce; Sigmar, Kristjan; Afreen, Esha; Nguyen, Tammy; Randolph, Amanda; Taber, Alexandria; Krzeszowski, Ashley; Robinett, Brittney; Volkin, David B.; Grassi, Fabio; Guerrant, Richard; Takeuchi, Ryo; Finrow, Brian; Behnke, Craig; Roberts, James (Nature Research, 2022-03-21)
    The use of the edible photosynthetic cyanobacterium Arthrospira platensis (spirulina) as a biomanufacturing platform has been limited by a lack of genetic tools. Here we report genetic engineering methods for stable, ...
  • Strategies to Screen Anti-AQP4 Antibodies from Yeast Surface Display Libraries 

    Huang, Aric; Jin, Wei; Fahad, Ahmed S.; Mussman, Brooklyn K.; Nicchia, Grazia Paola; Madan, Bharat; de Souza, Matheus Oliveira; Griffin, J. Daniel; Bennett, Jeffrey L.; Frigeri, Antonio; Berkland, Cory J.; DeKosky, Brandon J. (MDPI, 2022-06-05)
    A rapid and effective method to identify disease-specific antibodies from clinical patients is important for understanding autoimmune diseases and for the development of effective disease therapies. In neuromyelitis optica ...
  • Analytical and Preformulation Characterization Studies of Human Papillomavirus Virus-Like Particles to Enable Quadrivalent Multi-Dose Vaccine Formulation Development (Dataset) 

    Jerajani, Kaushal; Wan, Ying; Hickey, John M.; Kumru, Ozan S.; Sharma, Nitya; Pullagurla, Swathi R.; Ogun, Oluwadara; Mapari, Shweta; Whitaker, Neal; Brendle, Sarah; Christensen, Neil D.; Batwal, Saurabh; Mahedvi, Mustafa; Rao, Harish; Dogar, Vikas; Chandrasekharan, Rahul; Shaligram, Umesh; Joshi, Sangeeta B.; Volkin, David B. (Elsevier, 2022)
    Introducing multi-dose formulations of Human Papillomavirus (HPV) vaccines will reduce costs and enable improved global vaccine coverage, especially in low- and middle-income countries. This work describes the development ...
  • Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron 

    Teng, I-Ting; Nazzari, Alexandra F.; Choe, Misook; Liu, Tracy; de Souza, Matheus Oliveira; Petrova, Yuliya; Tsybovsky, Yaroslav; Wang, Shuishu; Zhang, Baoshan; Artamonov, Mykhaylo; Madan, Bharat; Huang, Aric; Lopez Acevedo, Sheila N.; Pan, Xiaoli; Ruckwardt, Tracy J.; DeKosky, Brandon J.; Mascola, John R.; Misasi, John; Sullivan, Nancy J.; Zhou, Tongqing; Kwong, Peter D. (Public Library of Science, 2022-05-24)
    Since the outbreak of the COVID-19 pandemic, widespread infections have allowed SARS-CoV-2 to evolve in human, leading to the emergence of multiple circulating variants. Some of these variants show increased resistance to ...
  • Antigen-adjuvant interactions, stability, and immunogenicity profiles of a SARS-CoV-2 receptor-binding domain (RBD) antigen formulated with aluminum salt and CpG adjuvants (Datasets) 

    Bajoria, Sakshi; Kaur, Kawaljit; Kumru, Ozan S.; Van Slyke, Greta; Doering, Jennifer; Novak, Hayley; Rodriguez-Aponte, Sergio A.; Dalvie, Neil C.; Naranjo, Christopher A.; Johnston, Ryan S.; Maxwell Silverman, Judith; Kleanthous, Harry; Love, J. Christopher; Mantis, Nicholas J.; Joshi, Sangeeta B.; Volkin, David B. (2022-05-17)
    Low-cost, refrigerator-stable COVID-19 vaccines will facilitate global access and improve vaccine coverage in low-and middle-income countries. To this end, subunit-based approaches targeting the receptor-binding domain ...
  • SARS-CoV-2 receptor binding domain displayed on HBsAg virus–like particles elicits protective immunity in macaques 

    Dalvie, Neil C.; Tostanoski, Lisa H.; Rodriguez-Aponte, Sergio A.; Kaur, Kawaljit; Bajoria, Sakshi; Kumru, Ozan S.; Martinot, Amanda J.; Chandrashekar, Abishek; McMahan, Katherine; Mercado, Noe B.; Yu, Jingyou; Chang, Aiquan; Giffin, Victoria M.; Nampanya, Felix; Patel, Shivani; Bowman, Lesley; Naranjo, Christopher A.; Yun, Dongsoo; Flinchbaugh, Zach; Pessaint, Laurent; Brown, Renita; Velasco, Jason; Teow, Elyse; Cook, Anthony; Andersen, Hanne; Lewis, Mark G.; Camp, Danielle L.; Silverman, Judith Maxwell; Nagar, Gaurav S.; Rao, Harish D.; Lothe, Rakesh R.; Chandrasekharan, Rahul; Rajurkar, Meghraj P.; Shaligram, Umesh S.; Kleanthous, Harry; Joshi, Sangeeta B.; Volkin, David B.; Biswas, Sumi; Love, J. Christopher; Barouch, Dan H. (American Association for the Advancement of Science, 2022-03-16)
    Authorized vaccines against SARS-CoV-2 remain less available in low- and middle-income countries due to insufficient supply, high costs, and storage requirements. Global immunity could still benefit from new vaccines using ...
  • Formulation development of a live attenuated human rotavirus (RV3-BB) vaccine candidate for use in low- and middle-income countries 

    Kumar, Prashant; Shukla, Ravi S.; Patel, Ashaben; Pullagurla, Swathi R.; Bird, Christopher; Ogun, Oluwadara; Kumru, Ozan S.; Hamidi, Ahd; Hoeksema, Femke; Yallop, Christopher; Bines, Julie E.; Joshi, Sangeeta B.; Volkin, David B. (Taylor and Francis, 2021-04-16)
    Formulation development was performed with the live, attenuated, human neonatal rotavirus vaccine candidate (RV3-BB) with three main objectives to facilitate use in low- and middle- income countries including (1) a liquid, ...
  • Intratumoral Cancer Chemotherapy with a Carrier-Based Immunogenic Cell-Death Eliciting Platinum (IV) Agent 

    Groer, Chad; Zhang, Ti; Lu, Ruolin; Cai, Shuang; Mull, Derek; Huang, Aric; Forrest, Melanie; Berkland, Cory; Aires, Daniel; Forrest, Marcus Laird (American Chemical Society, 2020-09-25)
    A carrier-based, immunogenic cell death (ICD)-eliciting platinum(IV) chemotherapeutic agent was synthesized via complexation between an axially derivatized Pt(IV)-tocopherol and hyaluronan (HA)–tocopherol nanocarrier. The ...
  • Development of a Broadly Protective, Self-Adjuvanting Subunit Vaccine to Prevent Infections by Pseudomonas aeruginosa 

    Das, Sayan; Howlader, Debaki R.; Zheng, Qi; Ratnakaram, Siva Sai Kumar; Whittier, Sean K.; Lu, Ti; Keith, Johnathan D.; Picking, William D.; Birket, Susan E.; Picking, Wendy L. (Frontiers Media, 2020-11-17)
    Infections caused by the opportunistic pathogen Pseudomonas aeruginosa can be difficult to treat due to innate and acquired antibiotic resistance and this is exacerbated by the emergence of multi-drug resistant strains. ...
  • Preformulation Characterization and the Effect of Ionic Excipients on the Stability of a Novel DB Fusion Protein 

    Jain, Akshay; Hu, Gang; Ratnakaram, Siva Sai Kumar; Johnson, David K.; Picking, William D.; Picking, Wendy L.; Middaugh, Charles Russell (Frontiers Media, 2021-01-01)
    Shigella ssp cause bacillary dysentery (shigellosis) which has high global morbidity in young children and the elderly. The virulence of Shigella relies upon a type III secretion system (T3SS) which injects host altering ...
  • Effect of Formulation Variables on the Stability of a Live, Rotavirus (RV3-BB) Vaccine Candidate using in vitro Gastric Digestion Models to Mimic Oral Delivery 

    Kumar, Prashant; Pullagurla, Swathi R.; Patel, Ashaben; Shukla, Ravi S.; Bird, Christopher; Kumru, Ozan S.; Hamidi, Ahd; Hoeksema, Femke; Yallop, Christopher; Bines, Julie E.; Joshi, Sangeeta B.; Volkin, David B. (Wiley, 2020-10-06)
    In this work, two different in vitro gastric digestion models were used to evaluate the stability of a live attenuated rotavirus vaccine candidate (RV3-BB) under conditions designed to mimic oral delivery in infants. First, ...
  • Synthetic Cationic Autoantigen Mimics Glatiramer Acetate Persistence at the Site of Injection and Is Efficacious Against Experimental Autoimmune Encephalomyelitis 

    Song, Jimmy Y.; Griffin, J. Daniel; Larson, Nicholas R.; Christopher, Matthew A.; Middaugh, C. Russell; Berkland, Cory J. (Frontiers Media, 2021-01-18)
    A synthetic peptide, K-PLP, consisting of 11-unit poly-lysine (K11) linked via polyethylene glycol (PEG) to proteolipid protein epitope (PLP) was synthesized, characterized, and evaluated for efficacy in ameliorating ...
  • Investigation of a monoclonal antibody against enterotoxigenic Escherichia coli, expressed as secretory IgA1 and IgA2 in plants 

    Teh, Audrey Y-H; Cavacini, Lisa; Hu, Yue; Kumru, Ozan S.; Xiong, Jian; Bolick, David T.; Joshi, Sangeeta B.; Grünwald-Gruber, Clemens; Altmann, Friedrich; Klempner, Mark; Guerrant, Richard L.; Volkin, David B.; Wang, Yang; Ma, Julian K-C. (Taylor & Francis Open Access, 2021-01-13)
    Passive immunization with antibodies is a promising approach against enterotoxigenic Escherichia coli diarrhea, a prevalent disease in LMICs. The objective of this study was to investigate expression of a monoclonal anti-ETEC ...
  • The Shigella Type III Secretion System: An Overview from Top to Bottom 

    Muthuramalingam, Meenakumari; Whittier, Sean K.; Picking, Wendy L.; Picking, William D. (MDPI, 2021-02-22)
    Shigella comprises four species of human-restricted pathogens causing bacillary dysentery. While Shigella possesses multiple genetic loci contributing to virulence, a type III secretion system (T3SS) is its primary virulence ...
  • Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients 

    Fahad, Ahmed S.; Timm, Morgan R.; Madan, Bharat; Burgomaster, Katherine E.; Dowd, Kimberly A.; Normandin, Erica; Gutiérrez-González, Matías F.; Pennington, Joseph M.; De Souza, Matheus Oliveira; Henry, Amy R.; Laboune, Farida; Wang, Lingshu; Ambrozak, David R.; Gordon, Ingelise J.; Douek, Daniel C.; Ledgerwood, Julie E.; Graham, Barney S.; Castilho, Leda R.; Pierson, Theodore C.; Mascola, John R.; DeKosky, Brandon J. (Frontiers Media, 2021-02-24)
    The re-emergence of Zika virus (ZIKV) caused widespread infections that were linked to Guillain-Barré syndrome in adults and congenital malformation in fetuses, and epidemiological data suggest that ZIKV infection can ...
  • Lung Surfactant Decreases Biochemical Alterations and Oxidative Stress Induced by a Sub-Toxic Concentration of Carbon Nanoparticles in Alveolar Epithelial and Microglial Cells 

    Caruso, Giuseppe; Fresta, Claudia G.; Costantino, Angelita; Lazzarino, Giacomo; Amorini, Angela M.; Lazzarino, Giuseppe; Tavazzi, Barbara; Lunte, Susan M.; Dhar, Prajnaparamita; Gulisano, Massimo; Caraci, Filippo (MDPI, 2021-03-07)
    Carbon-based nanomaterials are nowadays attracting lots of attention, in particular in the biomedical field, where they find a wide spectrum of applications, including, just to name a few, the drug delivery to specific ...
  • Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus 

    Dalvie, Neil C.; Brady, Joseph R.; Crowell, Laura E.; Tracey, Mary Kate; Biedermann, Andrew M.; Kaur, Kawaljit; Hickey, John M.; Kristensen, D. Lee, II; Bonnyman, Alexandra D.; Rodriguez-Aponte, Sergio A.; Whittaker, Charles A.; Bok, Marina; Vega, Celina; Mukhopadhyay, Tarit K.; Joshi, Sangeeta B.; Volkin, David B.; Parreño, Viviana; Love, Kerry R.; Love, J. Christopher (BMC, 2021-05-01)
    Background Vaccines comprising recombinant subunit proteins are well-suited to low-cost and high-volume production for global use. The design of manufacturing processes to produce subunit vaccines depends, however, on the ...
  • Carnosine Protects Macrophages against the Toxicity of Aβ1-42 Oligomers by Decreasing Oxidative Stress 

    Caruso, Giuseppe; Benatti, Cristina; Musso, Nicolò; Fresta, Claudia G.; Fidilio, Annamaria; Spampinato, Giorgia; Brunello, Nicoletta; Bucolo, Claudio; Drago, Filippo; Lunte, Susan M.; Peterson, Blake R.; Tascedda, Fabio; Caraci, Filippo (MDPI, 2021-04-26)
    Carnosine (β-alanyl-L-histidine) is a naturally occurring endogenous peptide widely distributed in excitable tissues such as the brain. This dipeptide has well-known antioxidant, anti-inflammatory, and anti-aggregation ...
  • Developing a manufacturing process to deliver a cost effective and stable liquid human rotavirus vaccine 

    Hamidi, Ahd; Hoeksema, Femke; Velthof, Pim; Lemckert, Angelique; Gillissen, Gert; Luitjens, Alfred; Bines, Julie E.; Pullagurla, Swathi R.; Kumar, Prashant; Volkin, David B.; Joshi, Sangeeta B.; Havenga, Menzo; Bakker, Wilfried A.M.; Yallop, Christopher (Elsevier, 2021-04-08)
    Despite solid evidence of the success of rotavirus vaccines in saving children from fatal gastroenteritis, more than 82 million infants worldwide still lack access to a rotavirus vaccine. The main barriers to global rotavirus ...
  • Sequence-Signature Optimization Enables Improved Identification of Human HV6-1-Derived Class Antibodies That Neutralize Diverse Influenza A Viruses 

    Chuang, Gwo-Yu; Shen, Chen-Hsiang; Cheung, Crystal Sao-Fong; Gorman, Jason; Creanga, Adrian; Joyce, M. Gordon; Leung, Kwanyee; Rawi, Reda; Wang, Lingshu; Yang, Eun Sung; Yang, Yongping; Zhang, Baoshan; Zhang, Yi; Kanekiyo, Masaru; Zhou, Tongqing; DeKosky, Brandon J.; Graham, Barney S.; Mascola, John R.; Kwong, Peter D. (Frontiers Media, 2021-05-31)
    Sequence signatures of multidonor broadly neutralizing influenza antibodies can be used to quantify the prevalence of B cells with virus-neutralizing potential to accelerate development of broadly protective vaccine ...

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