Show simple item record

dc.contributor.authorDeng, Lu
dc.contributor.authorShang, Li
dc.contributor.authorBai, Shoumin
dc.contributor.authorChen, Ji
dc.contributor.authorHe, Xueyan
dc.contributor.authorMartin-Trevino, Rachel
dc.contributor.authorChen, Shanshan
dc.contributor.authorLi, Xiao-yan
dc.contributor.authorMeng, Xiaojie
dc.contributor.authorYu, Bin
dc.contributor.authorWang, Xiaolin
dc.contributor.authorLiu, Yajing
dc.contributor.authorMcDermott, Sean P.
dc.contributor.authorAriazi, Alexa E.
dc.contributor.authorGinestier, Christophe
dc.contributor.authorIbarra, Ingrid
dc.contributor.authorKe, Jia
dc.contributor.authorLuther, Tahra
dc.contributor.authorClouthier, Shawn G.
dc.contributor.authorXu, Liang
dc.contributor.authorShan, Ge
dc.contributor.authorSong, Erwei
dc.contributor.authorYao, Heruui
dc.contributor.authorHannon, Gregory J.
dc.contributor.authorWeiss, Stephen J.
dc.contributor.authorWicha, Max S.
dc.contributor.authorLiu, Suling
dc.date.accessioned2017-05-09T18:51:43Z
dc.date.available2017-05-09T18:51:43Z
dc.date.issued2014-11-15
dc.identifier.citationDeng, L., Shang, L., Bai, S., Chen, J., He, X., Martin-Trevino, R., … Liu, S. (2014). MicroRNA100 Inhibits Self-Renewal of Breast Cancer Stem–like Cells and Breast Tumor Development. Cancer Research, 74(22), 6648–6660. http://doi.org/10.1158/0008-5472.CAN-13-3710en_US
dc.identifier.urihttp://hdl.handle.net/1808/24052
dc.description.abstractmiRNAs are essential for self-renewal and differentiation of normal and malignant stem cells by regulating the expression of key stem cell regulatory genes. Here, we report evidence implicating the miR100 in self-renewal of cancer stem-like cells (CSC). We found that miR100 expression levels relate to the cellular differentiation state, with lowest expression in cells displaying stem cell markers. Utilizing a tetracycline-inducible lentivirus to elevate expression of miR100 in human cells, we found that increasing miR100 levels decreased the production of breast CSCs. This effect was correlated with an inhibition of cancer cell proliferation in vitro and in mouse tumor xenografts due to attenuated expression of the CSC regulatory genes SMARCA5, SMARCD1, and BMPR2. Furthermore, miR100 induction in breast CSCs immediately upon their orthotopic implantation or intracardiac injection completely blocked tumor growth and metastasis formation. Clinically, we observed a significant association between miR100 expression in breast cancer specimens and patient survival. Our results suggest that miR100 is required to direct CSC self-renewal and differentiation.en_US
dc.publisherAmerican Association for Cancer Researchen_US
dc.rights©2014 American Association for Cancer Research.en_US
dc.titleMicroRNA100 Inhibits Self-Renewal of Breast Cancer Stem–like Cells and Breast Tumor Developmenten_US
dc.typeArticleen_US
kusw.kuauthorMeng, Xiaojie
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1158/0008-5472.CAN-13-3710en_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC4370193en_US
dc.rights.accessrightsopenAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record