Calcium-crosslinked LABL-TAT complexes effectively target gene delivery to ICAM-1 expressing cells
Baoum, Abdulgader Ahmed
Siahaan, Teruna J.
Berkland, Cory J.
American Chemical Society
Scholarly/refereed, author accepted manuscript
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Molecular Pharmaceutics, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/mp100393j.
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Targeted gene delivery using non-viral vectors is a highly touted scheme to reduce the potential for toxic or immunological side effects by reducing dose. In previous reports, TAT polyplexes with DNA have shown relatively poor gene delivery. The transfection efficiency has been enhanced by condensing TAT/DNA complexes to a small particle size using calcium. To explore the targetability of these condensed TAT complexes, LABL peptide targeting intercellular cell-adhesion molecule-1 (ICAM-1) was conjugated to TAT peptide using a polyethylene glycol (PEG) spacer. PEGylation reduced the transfection efficiency of TAT, but TAT complexes targeting ICAM-1 expressing cells regained much of the lost transfection efficiency. Targeted block peptides properly formulated with calcium offer promise for gene delivery to ICAM-1 expressing cells at sites of injury or inflammation.
Khondee, S., Baoum, A., Siahaan, T. J., & Berkland, C. (2011). Calcium-crosslinked LABL-TAT complexes effectively target gene delivery to ICAM-1 expressing cells. Molecular Pharmaceutics, 8(3), 788–798. http://doi.org/10.1021/mp100393j
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