dc.contributor.author | Khondee, Supang | |
dc.contributor.author | Baoum, Abdulgader Ahmed | |
dc.contributor.author | Siahaan, Teruna J. | |
dc.contributor.author | Berkland, Cory J. | |
dc.date.accessioned | 2017-05-08T18:09:38Z | |
dc.date.available | 2017-05-08T18:09:38Z | |
dc.date.issued | 2011-06-06 | |
dc.identifier.citation | Khondee, S., Baoum, A., Siahaan, T. J., & Berkland, C. (2011). Calcium-crosslinked LABL-TAT complexes effectively target gene delivery to ICAM-1 expressing cells. Molecular Pharmaceutics, 8(3), 788–798. http://doi.org/10.1021/mp100393j | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24016 | |
dc.description.abstract | Targeted gene delivery using non-viral vectors is a highly touted scheme to reduce the potential for toxic or immunological side effects by reducing dose. In previous reports, TAT polyplexes with DNA have shown relatively poor gene delivery. The transfection efficiency has been enhanced by condensing TAT/DNA complexes to a small particle size using calcium. To explore the targetability of these condensed TAT complexes, LABL peptide targeting intercellular cell-adhesion molecule-1 (ICAM-1) was conjugated to TAT peptide using a polyethylene glycol (PEG) spacer. PEGylation reduced the transfection efficiency of TAT, but TAT complexes targeting ICAM-1 expressing cells regained much of the lost transfection efficiency. Targeted block peptides properly formulated with calcium offer promise for gene delivery to ICAM-1 expressing cells at sites of injury or inflammation. | en_US |
dc.publisher | American Chemical Society | en_US |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Molecular Pharmaceutics, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/mp100393j. | en_US |
dc.subject | TAT | en_US |
dc.subject | LABL | en_US |
dc.subject | Targeted gene delivery | en_US |
dc.subject | Calcium crosslinking | en_US |
dc.subject | ICAM-1 | en_US |
dc.title | Calcium-crosslinked LABL-TAT complexes effectively target gene delivery to ICAM-1 expressing cells | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Khondee, Supang | |
kusw.kuauthor | Baoum, Abdulgader Ahmed | |
kusw.kuauthor | Siahaan, Teruna J. | |
kusw.kuauthor | Berkland, Cory J. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
kusw.oanotes | Per SHERPA/RoMEO 5/8/2017: Author's Pre-print: grey tick subject to Restrictions below, author can archive pre-print (ie pre-refereeing)
Restrictions: Must obtain written permission from Editor
Must not violate ACS ethical GuidelinesAuthor's Post-print: grey tick subject to Restrictions below, author can archive post-print (ie final draft post-refereeing)
Restrictions: If mandated by funding agency or employer/ institution
If mandated to deposit before 12 months, must obtain waiver from Institution/Funding agency or use AuthorChoice
12 months embargoPublisher's Version/PDF: cross author cannot archive publisher's version/PDF
General Conditions: On author's personal website, pre-print servers, institutional website, institutional repositories or subject repositories
Non-Commercial
Must be accompanied by set statement (see policy)
Must link to publisher version
Publisher's version/PDF cannot be used | en_US |
dc.identifier.doi | 10.1021/mp100393j | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC4207658 | en_US |
dc.rights.accessrights | openAccess | |