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dc.contributor.authorZhang, Lisha
dc.contributor.authorNebane, N. Miranda
dc.contributor.authorWennerberg, Krister
dc.contributor.authorLi, Yujie
dc.contributor.authorNeubauer, Valerie
dc.contributor.authorHobarth, Judith V.
dc.contributor.authorMcKellip, Sara N.
dc.contributor.authorRasmussen, Lynn
dc.contributor.authorShindo, Nice
dc.contributor.authorSosa, Melinda
dc.contributor.authorMaddry, Joseph A.
dc.contributor.authorAnanthan, Subramaniam
dc.contributor.authorPiazza, Gary A.
dc.contributor.authorWhite, E. Lucile
dc.contributor.authorHarsay, Edina
dc.date.accessioned2017-05-08T17:37:34Z
dc.date.available2017-05-08T17:37:34Z
dc.date.issued2010-06-14
dc.identifier.citationZhang, L., Nebane, N. M., Wennerberg, K., Li, Y., Neubauer, V., Hobrath, J. V., … Harsay, E. (2010). A high-throughput screen for chemical inhibitors of exocytic transport in yeast. Chembiochem : A European Journal of Chemical Biology, 11(9), 1291–1301. http://doi.org/10.1002/cbic.200900681en_US
dc.identifier.urihttp://hdl.handle.net/1808/24012
dc.descriptionThis is the peer reviewed version of the following article: Zhang, L., Nebane, N. M., Wennerberg, K., Li, Y., Neubauer, V., Hobrath, J. V., … Harsay, E. (2010). A high-throughput screen for chemical inhibitors of exocytic transport in yeast. Chembiochem : A European Journal of Chemical Biology, 11(9), 1291–1301. http://doi.org/10.1002/cbic.200900681, which has been published in final form at doi.org/10.1002/cbic.200900681. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en_US
dc.description.abstractMost of the components of the membrane and protein traffic machinery were discovered by perturbing their functions, either with bioactive compounds or by mutations. However, the mechanisms responsible for exocytic transport vesicle formation at the Golgi and endosomes are still largely unknown. Both the exocytic traffic routes and the signaling pathways that regulate these routes are highly complex and robust, so that defects can be overcome by alternate pathways or mechanisms. A classical yeast genetic screen designed to account for the robustness of the exocytic pathway identified a novel conserved gene, AVL9, that functions in late exocytic transport. We now describe a chemical-genetic version of the mutant screen, in which we performed a high-throughput phenotypic screen of a large compound library and identified novel small molecule secretory inhibitors. In order to maximize the number and diversity of our hits, the screen was performed in a pdr5Δ snq2Δ mutant background, which lacks two transporters responsible for pleiotropic drug resistance. However, we found that deletion of both transporters reduced the fitness of our screen strain, whereas the pdr5Δ mutation had relatively small effect on growth and was also the more important transporter mutation for conferring sensitivity to our hits. In this and similar chemical-genetic yeast screens, using just a single pump mutation may be sufficient for increasing hit diversity while minimizing the physiological effects of transporter mutations.en_US
dc.publisherWileyen_US
dc.rights© 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen_US
dc.subjectAntifungal agentsen_US
dc.subjectBiological activityen_US
dc.subjectChemical geneticsen_US
dc.subjectInhibitorsen_US
dc.subjectProtein trafficen_US
dc.titleA high-throughput screen for chemical inhibitors of exocytic transport in yeasten_US
dc.typeArticleen_US
kusw.kuauthorZhang, Lisha
kusw.kuauthorLi, Yujie
kusw.kuauthorNeubauer, Valerie
kusw.kuauthorHarsay, Edina
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1002/cbic.200900681en_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC3090732en_US
dc.rights.accessrightsopenAccess


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