dc.contributor.author | Gunasekara, Dulan B. | |
dc.contributor.author | Hulvey, Matthew K. | |
dc.contributor.author | Lunte, Susan M. | |
dc.contributor.author | da Silva, José A. F. | |
dc.date.accessioned | 2017-03-31T20:30:12Z | |
dc.date.available | 2017-03-31T20:30:12Z | |
dc.date.issued | 2012-03-14 | |
dc.identifier.citation | Gunasekara, D. B., Hulvey, M. K., Lunte, S. M., & da Silva, J. A. F. (2012). Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts. Analytical and Bioanalytical Chemistry, 403(8), 2377–2384. http://doi.org/10.1007/s00216-012-5810-4 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23560 | |
dc.description.abstract | Microchip electrophoresis (ME) with electrochemical detection was used to monitor nitric oxide (NO) production from diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) and 1-(hydroxyl-NNO-azoxy)-L-proline disodium salt (PROLI/NO). NO was generated through acid hydrolysis of these NONOate salts. The products of acid hydrolysis were introduced into a 5-cm separation channel using gated injection. The separation was accomplished using reverse polarity and a background electrolyte consisting of 10 mM boric acid and 2 mM tetradecyltrimethylammonium bromide, pH 11. Electrochemical detection was performed using an isolated potentiostat in an in-channel configuration. Potentials applied to the working electrode, typically higher than +1.0 V vs. Ag/AgCl, allowed the direct detection of nitrite, NO, DEA/NO, and PROLI/NO. Baseline resolution was achieved for the separation of PROLI/NO and NO while resolution between DEA/NO and NO was poor (1.0±0.2). Nitrite was present in all samples tested. | en_US |
dc.publisher | Springer Verlag | en_US |
dc.rights | © Springer-Verlag 2012 | en_US |
dc.subject | Nitric oxide | en_US |
dc.subject | Microchip electrophoresis | en_US |
dc.subject | Reactive nitrogen species | en_US |
dc.subject | Amperometric detection | en_US |
dc.subject | NONOates | en_US |
dc.title | Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Gunasekara, Dulan B. | |
kusw.kuauthor | Lunte, Susan M. | |
kusw.kudepartment | Chemistry | en_US |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1007/s00216-012-5810-4 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-9522-6181 | |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess | |