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dc.contributor.authorGunasekara, Dulan B.
dc.contributor.authorHulvey, Matthew K.
dc.contributor.authorLunte, Susan M.
dc.contributor.authorda Silva, José A. F.
dc.date.accessioned2017-03-31T20:30:12Z
dc.date.available2017-03-31T20:30:12Z
dc.date.issued2012-03-14
dc.identifier.citationGunasekara, D. B., Hulvey, M. K., Lunte, S. M., & da Silva, J. A. F. (2012). Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts. Analytical and Bioanalytical Chemistry, 403(8), 2377–2384. http://doi.org/10.1007/s00216-012-5810-4en_US
dc.identifier.urihttp://hdl.handle.net/1808/23560
dc.description.abstractMicrochip electrophoresis (ME) with electrochemical detection was used to monitor nitric oxide (NO) production from diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) and 1-(hydroxyl-NNO-azoxy)-L-proline disodium salt (PROLI/NO). NO was generated through acid hydrolysis of these NONOate salts. The products of acid hydrolysis were introduced into a 5-cm separation channel using gated injection. The separation was accomplished using reverse polarity and a background electrolyte consisting of 10 mM boric acid and 2 mM tetradecyltrimethylammonium bromide, pH 11. Electrochemical detection was performed using an isolated potentiostat in an in-channel configuration. Potentials applied to the working electrode, typically higher than +1.0 V vs. Ag/AgCl, allowed the direct detection of nitrite, NO, DEA/NO, and PROLI/NO. Baseline resolution was achieved for the separation of PROLI/NO and NO while resolution between DEA/NO and NO was poor (1.0±0.2). Nitrite was present in all samples tested.en_US
dc.publisherSpringer Verlagen_US
dc.rights© Springer-Verlag 2012en_US
dc.subjectNitric oxideen_US
dc.subjectMicrochip electrophoresisen_US
dc.subjectReactive nitrogen speciesen_US
dc.subjectAmperometric detectionen_US
dc.subjectNONOatesen_US
dc.titleMicrochip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate saltsen_US
dc.typeArticleen_US
kusw.kuauthorGunasekara, Dulan B.
kusw.kuauthorLunte, Susan M.
kusw.kudepartmentChemistryen_US
kusw.kudepartmentPharmaceutical Chemistryen_US
dc.identifier.doi10.1007/s00216-012-5810-4en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-9522-6181
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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