Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts
Issue Date
2012-03-14Author
Gunasekara, Dulan B.
Hulvey, Matthew K.
Lunte, Susan M.
da Silva, José A. F.
Publisher
Springer Verlag
Type
Article
Article Version
Scholarly/refereed, author accepted manuscript
Rights
© Springer-Verlag 2012
Metadata
Show full item recordAbstract
Microchip electrophoresis (ME) with electrochemical detection was used to monitor nitric oxide (NO) production from diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) and 1-(hydroxyl-NNO-azoxy)-L-proline disodium salt (PROLI/NO). NO was generated through acid hydrolysis of these NONOate salts. The products of acid hydrolysis were introduced into a 5-cm separation channel using gated injection. The separation was accomplished using reverse polarity and a background electrolyte consisting of 10 mM boric acid and 2 mM tetradecyltrimethylammonium bromide, pH 11. Electrochemical detection was performed using an isolated potentiostat in an in-channel configuration. Potentials applied to the working electrode, typically higher than +1.0 V vs. Ag/AgCl, allowed the direct detection of nitrite, NO, DEA/NO, and PROLI/NO. Baseline resolution was achieved for the separation of PROLI/NO and NO while resolution between DEA/NO and NO was poor (1.0±0.2). Nitrite was present in all samples tested.
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Citation
Gunasekara, D. B., Hulvey, M. K., Lunte, S. M., & da Silva, J. A. F. (2012). Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts. Analytical and Bioanalytical Chemistry, 403(8), 2377–2384. http://doi.org/10.1007/s00216-012-5810-4
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