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dc.contributor.authorMattingly, Brendan Christopher
dc.contributor.authorBuechner, Matthew
dc.date.accessioned2017-03-06T22:45:26Z
dc.date.available2017-03-06T22:45:26Z
dc.date.issued2012-11-01
dc.identifier.citationMattingly, Brendan C., and Matthew Buechner. "The FGD Homologue EXC-5 Regulates Apical Trafficking in C. Elegans Tubules." Developmental Biology 359.1 (2011): 59-72.en_US
dc.identifier.urihttp://hdl.handle.net/1808/23345
dc.description.abstractMaintenance of the shape of biological tubules is critical for development and physiology of metazoan organisms. Loss of function of the C. elegans FGD protein EXC-5 allows large fluid-filled cysts to form in the lumen of the single-cell excretory canal tubules, while overexpression of exc-5 causes defects at the tubule’s basolateral surface. We have examined the effects of altering expression levels of exc-5 on the distribution of fluorescently-marked subcellular organelles. In exc-5 mutants, early endosomes build up in the cell, especially in areas close to cysts, while recycling endosomes are depleted. Endosome morphology changes prior to cyst formation. Conversely, when exc-5 is overexpressed, recycling endosomes are enriched. Since FGD proteins activate the small GTPases CDC42 and Rac, these results support the hypothesis that EXC-5 acts through small GTPases to move material from apical early endosomes to recycling endosomes, and that loss of such movement is likely the cause of tubule deformation both in nematodes and in tissues affected by FGD dysfunction such as Charcot-Marie-Tooth Syndrome type 4H.en_US
dc.publisherElsevieren_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subjectCharcot-Marie-Tooth Syndromeen_US
dc.subjectFGDen_US
dc.subjectEEA1en_US
dc.subjectRME-1en_US
dc.subjectRaben_US
dc.subjectCDC42en_US
dc.subjectEndosome recyclingen_US
dc.titleThe FGD Homologue EXC-5 Regulates Apical Trafficking in C. elegans Tubulesen_US
dc.typeArticleen_US
kusw.kuauthorBuechner, Matthew
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1016/j.ydbio.2011.08.011en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-2103-9399
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Except where otherwise noted, this item's license is described as: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License 3.0 (CC BY-NC-ND 3.0 US), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.