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dc.contributor.authorCreamer, Jessica Stephens
dc.contributor.authorOborny, Nathan John
dc.contributor.authorLunte, Susan M.
dc.date.accessioned2017-01-12T19:36:44Z
dc.date.available2017-01-12T19:36:44Z
dc.date.issued2015-07-08
dc.identifier.citationCreamer, Jessica S., Nathan J. Oborny, and Susan M. Lunte. "Recent Advances in the Analysis of Therapeutic Proteins by Capillary and Microchip Electrophoresis." Analytical Methods 6.15 (2014): 5427.en_US
dc.identifier.urihttp://hdl.handle.net/1808/22619
dc.description.abstractThe development of therapeutic proteins and peptides is an expensive and time-intensive process. Biologics, which have become a multi-billion dollar industry, are chemically complex products that require constant observation during each stage of development and production. Post-translational modifications along with chemical and physical degradation from oxidation, deamidation, and aggregation, lead to high levels of heterogeneity that affect drug quality and efficacy. The various separation modes of capillary electrophoresis (CE) are commonly utilized to perform quality control and assess protein heterogeneity. This review attempts to highlight the most recent developments and applications of CE separation techniques for the characterization of protein and peptide therapeutics by focusing on papers accepted for publication in the in the two-year period between January 2012 and December 2013. The separation principles and technological advances of CE, capillary gel electrophoresis, capillary isoelectric focusing, capillary electrochromatography and CE-mass spectrometry are discussed, along with exciting new applications of these techniques to relevant pharmaceutical issues. Also included is a small selection of papers on microchip electrophoresis to show the direction this field is moving with regards to the development of inexpensive and portable analysis systems for on-site, high-throughput analysis.en_US
dc.publisherRoyal Society of Chemistryen_US
dc.titleRecent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresisen_US
dc.typeArticleen_US
kusw.kuauthorLunte, Susan M.
kusw.kudepartmentChemistryen_US
kusw.oanotesPer SHERPA/RoMEO 1/12/2017: Author's Pre-print: green tick author can archive pre-print (ie pre-refereeing) Author's Post-print: grey tick subject to Restrictions below, author can archive post-print (ie final draft post-refereeing) Restrictions: 12 months embargo Publisher's Version/PDF: cross author cannot archive publisher's version/PDF General Conditions: Pre-prints on non-commercial repositories and arXiv Author's post-print on author's personal website, institutional repository, open access repository, scholarly communications networks that comply with International Association of STM Publishers sharing principles Publisher's version/PDF cannot be used Restrictions on further re-use and further distribution to be noted Publisher will deposit in Chemical Sciences Article Repository if requested NIH authors may have their articles deposited in PubMed Central on their behalf.en_US
dc.identifier.doi10.1039/C4AY00447Gen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-2890-4901
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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