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    Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

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    Issue Date
    2015-07-08
    Author
    Creamer, Jessica Stephens
    Oborny, Nathan John
    Lunte, Susan M.
    Publisher
    Royal Society of Chemistry
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
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    Abstract
    The development of therapeutic proteins and peptides is an expensive and time-intensive process. Biologics, which have become a multi-billion dollar industry, are chemically complex products that require constant observation during each stage of development and production. Post-translational modifications along with chemical and physical degradation from oxidation, deamidation, and aggregation, lead to high levels of heterogeneity that affect drug quality and efficacy. The various separation modes of capillary electrophoresis (CE) are commonly utilized to perform quality control and assess protein heterogeneity. This review attempts to highlight the most recent developments and applications of CE separation techniques for the characterization of protein and peptide therapeutics by focusing on papers accepted for publication in the in the two-year period between January 2012 and December 2013. The separation principles and technological advances of CE, capillary gel electrophoresis, capillary isoelectric focusing, capillary electrochromatography and CE-mass spectrometry are discussed, along with exciting new applications of these techniques to relevant pharmaceutical issues. Also included is a small selection of papers on microchip electrophoresis to show the direction this field is moving with regards to the development of inexpensive and portable analysis systems for on-site, high-throughput analysis.
    URI
    http://hdl.handle.net/1808/22619
    DOI
    https://doi.org/10.1039/C4AY00447G
    Collections
    • Chemistry Scholarly Works [601]
    Citation
    Creamer, Jessica S., Nathan J. Oborny, and Susan M. Lunte. "Recent Advances in the Analysis of Therapeutic Proteins by Capillary and Microchip Electrophoresis." Analytical Methods 6.15 (2014): 5427.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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