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    Oligopolyphenylenevinylene-Conjugated Oligoelectrolyte Membrane Insertion Molecules Selectively Disrupt Cell Envelopes of Gram-Positive Bacteria

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    HancockLE_2015.pdf (2.009Mb)
    Issue Date
    2015-01-09
    Author
    Hinks, Jamie
    Poh, Wee Han
    Chu, Justin Jang Hann
    Loo, Joachim Say Chye
    Bazan, Guillermo C.
    Hancock, Lynn E.
    Wuertz, Stefan
    Publisher
    American Society for Microbiology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Rights
    Copyright © 2015, American Society for Microbiology. All Rights Reserved.
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    Abstract
    The modification of microbial membranes to achieve biotechnological strain improvement with exogenous small molecules, such as oligopolyphenylenevinylene-conjugated oligoelectrolyte (OPV-COE) membrane insertion molecules (MIMs), is an emerging biotechnological field. Little is known about the interactions of OPV-COEs with their target, the bacterial envelope. We studied the toxicity of three previously reported OPV-COEs with a selection of Gram-negative and Gram-positive organisms and demonstrated that Gram-positive bacteria are more sensitive to OPV-COEs than Gram-negative bacteria. Transmission electron microscopy demonstrated that these MIMs disrupt microbial membranes and that this occurred to a much greater degree in Gram-positive organisms. We used a number of mutants to probe the nature of MIM interactions with the microbial envelope but were unable to align the membrane perturbation effects of these compounds to previously reported membrane disruption mechanisms of, for example, cationic antimicrobial peptides. Instead, the data support the notion that OPV-COEs disrupt microbial membranes through a suspected interaction with diphosphatidylglycerol (DPG), a major component of Gram-positive membranes. The integrity of model membranes containing elevated amounts of DPG was disrupted to a greater extent by MIMs than those prepared from Escherichia coli total lipid extracts alone.
    URI
    http://hdl.handle.net/1808/22136
    DOI
    https://doi.org/10.1128/AEM.03355-14
    Collections
    • Molecular Biosciences Scholarly Works [528]
    Citation
    Hinks, J., Poh, W. H., Chu, J. J. H., Loo, J. S. C., Bazan, G. C., Hancock, L. E., & Wuertz, S. (2015). Oligopolyphenylenevinylene-Conjugated Oligoelectrolyte Membrane Insertion Molecules Selectively Disrupt Cell Envelopes of Gram-Positive Bacteria. Appl. Environ. Microbiol., 81(6), 1949–1958. doi:10.1128/aem.03355-14

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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