Interaction of Shigella flexneri IpaC with Model Membranes Correlates with Effects on Cultured Cells

View/ Open
Issue Date
2000-06Author
Tran, Ngoc
Barnoski Serfis, Alexa
Osiecki, John C.
Picking, Wendy Lynn
Coye, Lisette
Davis, Rebecca
Picking, William D.
Publisher
American Society for Microbiology
Type
Article
Article Version
Scholarly/refereed, publisher version
Published Version
http://iai.asm.org/content/68/6/3710.full?sid=def13237-1346-4e93-9913-9c36b1ec5cb0Rights
Copyright © 2000, American Society for Microbiology
Metadata
Show full item recordAbstract
Invasion of enterocytes by Shigella flexneri requires the properly timed release of IpaB and IpaC at the host-pathogen interface; however, only IpaC has been found to possess quantifiable activities in vitro. We demonstrate here that when added to cultured cells, purified IpaC elicits cytoskeletal changes similar to those that occur during Shigella invasion. This IpaC effect may correlate with its ability to interact with model membranes at physiological pH and to promote entry by an ipaC mutant of S. flexneri.
Collections
Citation
Tran, N., Serfis, A. B., Osiecki, J. C., Picking, W. L., Coye, L., Davis, R., & Picking, W. D. (2000). Interaction of Shigella flexneri IpaC with Model Membranes Correlates with Effects on Cultured Cells. Infection and Immunity, 68(6), 3710–3715.
Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.