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    In vivo photoacoustic imaging of chemotherapy-induced apoptosis in squamous cell carcinoma using a near-infrared caspase-9 probe

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    Yang_apoptosis2011.pdf (823.4Kb)
    Issue Date
    2011-10-27
    Author
    Yang, Qiuhong
    Cui, Huizhong
    Cai, Shuang
    Yang, Xinmai
    Forrest, M. Laird
    Publisher
    Society of Photo-optical Instrumentation Engineers (SPIE)
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    Anti-cancer drugs typically exert their pharmacological effect on tumors by inducing apoptosis, or programmed cell death, within the cancer cells. However, no tools exist in the clinic for detecting apoptosis in real time. Microscopic examination of surgical biopsies and secondary responses, such as morphological changes, are used to verify efficacy of a treatment. Here, we developed a novel near-infrared dye-based imaging probe to directly detect apoptosis with high specificity in cancer cells by utilizing a noninvasive photoacoustic imaging (PAI) technique. Nude mice bearing head and neck tumors received cisplatin chemotherapy (10 mg/kg) and were imaged by PAI after tail vein injection of the contrast agent. In vivo PAI indicated a strong apoptotic response to chemotherapy on the peripheral margins of tumors, whereas untreated controls showed no contrast enhancement by PAI. The apoptotic status of the mouse tumor tissue was verified by immunohistochemical techniques staining for cleaved caspase-3 p11 subunit. The results demonstrated the potential of this imaging probe to guide the evaluation of chemotherapy treatment.
    Description
    This is the published version. Copyright 2011 Society of Photo-optical Instrumentation Engineers (SPIE)
    URI
    http://hdl.handle.net/1808/18988
    DOI
    https://doi.org/10.1117/1.3650240
    Collections
    • Bioengineering Scholarly Works [127]
    • Mechanical Engineering Scholarly Works [114]
    Citation
    Yang, Qiuhong, Huizhong Cui, Shuang Cai, Xinmai Yang, and M. Laird Forrest. "In Vivo Photoacoustic Imaging of Chemotherapy-induced Apoptosis in Squamous Cell Carcinoma Using a Near-infrared Caspase-9 Probe." J. Biomed. Opt. Journal of Biomedical Optics 16.11 (2011): 116026. http://dx.doi.org/10.1117/1.3650240

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    KU Libraries
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    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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