Direct interaction of ligand–receptor pairs specifying stomatal patterning
Lee, Jin Suk
Kanaoka, Masahiro M.
McAbee, Jessica M.
Cold Spring Harbor Laboratory Press
Scholarly/refereed, publisher version
MetadataShow full item record
Valves on the plant epidermis called stomata develop according to positional cues, which likely involve putative ligands (EPIDERMAL PATTERNING FACTORS [EPFs]) and putative receptors (ERECTA family receptor kinases and TOO MANY MOUTHS [TMM]) in Arabidopsis. Here we report the direct, robust, and saturable binding of bioactive EPF peptides to the ERECTA family. In contrast, TMM exhibits negligible binding to EPF1 but binding to EPF2. The ERECTA family forms receptor homomers in vivo. On the other hand, TMM associates with the ERECTA family but not with itself. While ERECTA family receptor kinases exhibit complex redundancy, blocking ERECTA and ERECTA-LIKE1 (ERL1) signaling confers specific insensitivity to EPF2 and EPF1, respectively. Our results place the ERECTA family as the primary receptors for EPFs with TMM as a signal modulator and establish EPF2–ERECTA and EPF1–ERL1 as ligand–receptor pairs specifying two steps of stomatal development: initiation and spacing divisions.
This is the published version. Copyright © 2012 by Cold Spring Harbor Laboratory Press
Lee, J. S., T. Kuroha, M. Hnilova, D. Khatayevich, M. M. Kanaoka, J. M. Mcabee, M. Sarikaya, C. Tamerler, and K. U. Torii. "Direct Interaction of Ligand-receptor Pairs Specifying Stomatal Patterning." Genes & Development 26.2 (2012): 126-36. http://dx.doi.org/10.1101/gad.179895.111
Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.