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    Analysis of the Amino Acid Sequence Specificity Determinants of the Enterococcal cCF10 Sex Pheromone in Interactions with the Pheromone-Sensing Machinery

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    Fixen_amino_acid_sequence2006.pdf (213.8Kb)
    Issue Date
    2007-02
    Author
    Fixen, Kathryn R.
    Chandler, Josephine R.
    Le, Thinh
    Kozlowicz, Briana K.
    Manias, Dawn A.
    Dunny, Gary M.
    Publisher
    American Society for Microbiology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    The level of expression of conjugation genes in Enterococcus faecalis strains carrying the pheromone-responsive transferable plasmid pCF10 is determined by the ratio in the culture medium of two types of signaling peptides, a pheromone (cCF10) and an inhibitor (iCF10). Recent data have demonstrated that both peptides target the cytoplasmic receptor protein PrgX. However, the relative importance of the interaction of these peptides with the pCF10 protein PrgZ (which enhances import of cCF10) versus PrgX is not fully understood, and there is relatively little information about specific amino acid sequence determinants affecting the functional interactions of cCF10 with these proteins in vivo. To address these issues, we used a pheromone-inducible reporter gene system where various combinations of PrgX and PrgZ could be expressed in an isogenic host background to examine the biological activities of cCF10, iCF10, and variants of cCF10 isolated in a genetic screen. The results suggest that most of the amino acid sequence determinants of cCF10 pheromone activity affect interactions between the peptide and PrgX, although some sequence variants that affected peptide/PrgZ interactions were also identified. The results provide functional data to complement ongoing structural studies of PrgX and increase our understanding of the functional interactions of cCF10 and iCF10 with the pheromone-sensing machinery of pCF10.
    Description
    This is the published version. Copyright 2006 American Society for Microbiology
    URI
    http://hdl.handle.net/1808/18864
    DOI
    https://doi.org/10.1128/JB.01226-06
    Collections
    • Molecular Biosciences Scholarly Works [581]
    Citation
    Fixen, K. R., J. R. Chandler, T. Le, B. K. Kozlowicz, D. A. Manias, and G. M. Dunny. "Analysis of the Amino Acid Sequence Specificity Determinants of the Enterococcal CCF10 Sex Pheromone in Interactions with the Pheromone-Sensing Machinery." Journal of Bacteriology 189.4 (2006): 1399-406. http://dx.doi.org/10.1128/JB.01226-06

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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