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    Quorum-Sensing Control of Antibiotic Synthesis in Burkholderia thailandensis

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    Duerkop_quorum-sensing_control2009.pdf (477.0Kb)
    Issue Date
    2009-04-17
    Author
    Duerkop, Breck A.
    Varga, John
    Chandler, Josephine R.
    Peterson, Snow Brook
    Herman, Jake P.
    Churchill, Mair E. A.
    Parsek, Matthew R.
    Nierman, William C.
    Greenberg, E. Peter
    Publisher
    American Society for Microbiology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    The genome of Burkholderia thailandensis codes for several LuxR-LuxI quorum-sensing systems. We used B. thailandensis quorum-sensing deletion mutants and recombinant Escherichia coli to determine the nature of the signals produced by one of the systems, BtaR2-BtaI2, and to show that this system controls genes required for the synthesis of an antibiotic. BtaI2 is an acyl-homoserine lactone (acyl-HSL) synthase that produces two hydroxylated acyl-HSLs, N-3-hydroxy-decanoyl-HSL (3OHC10-HSL) and N-3-hydroxy-octanoyl-HSL (3OHC8-HSL). The btaI2 gene is positively regulated by BtaR2 in response to either 3OHC10-HSL or 3OHC8-HSL. The btaR2-btaI2 genes are located within clusters of genes with annotations that suggest they are involved in the synthesis of polyketide or peptide antibiotics. Stationary-phase cultures of wild-type B. thailandensis, but not a btaR2 mutant or a strain deficient in acyl-HSL synthesis, produced an antibiotic effective against gram-positive bacteria. Two of the putative antibiotic synthesis gene clusters require BtaR2 and either 3OHC10-HSL or 3OHC8-HSL for activation. This represents another example where antibiotic synthesis is controlled by quorum sensing, and it has implications for the evolutionary divergence of B. thailandensis and its close relatives Burkholderia pseudomallei and Burkholderia mallei.
    Description
    This is the published version. Copyright 2009 American Society for Microbiology
    URI
    http://hdl.handle.net/1808/18862
    DOI
    https://doi.org/10.1128/JB.00200-09
    Collections
    • Molecular Biosciences Scholarly Works [606]
    Citation
    Duerkop, B. A., J. Varga, J. R. Chandler, S. B. Peterson, J. P. Herman, M. E. A. Churchill, M. R. Parsek, W. C. Nierman, and E. P. Greenberg. "Quorum-Sensing Control of Antibiotic Synthesis in Burkholderia Thailandensis." Journal of Bacteriology 191.12 (2009): 3909-918. http://dx.doi.org/10.1128/JB.00200-09

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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