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    Quorum Sensing Protects Pseudomonas aeruginosa against Cheating by Other Species in a Laboratory Coculture Model

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    Smalley_quorum_sensing2015.pdf (611.5Kb)
    Issue Date
    2015-10
    Author
    Smalley, Nicole E.
    An, Dingding
    Parsek, Matthew R.
    Chandler, Josephine R.
    Dandekar, Ajai A.
    Publisher
    American Society for Microbiology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    Many species of bacteria use a cell-cell communication system called quorum sensing (QS) to coordinate group activities. QS systems frequently regulate the production of exoproducts. Some of these products, such as proteases, are “public goods” that are shared among the population and vulnerable to cheating by nonproducing members of the population. Because the QS system of the opportunistic pathogen Pseudomonas aeruginosa regulates several public goods, it can serve as a model for studying cooperation. Bacteria also commonly regulate antimicrobial production through QS. In this study, we focused on the hypothesis that QS-regulated antimicrobials may be important for P. aeruginosa to protect against cheating by another bacterial species, Burkholderia multivorans. We assessed laboratory cocultures of P. aeruginosa and B. multivorans and investigated the importance of three P. aeruginosa QS-regulated antimicrobials, hydrogen cyanide, rhamnolipids, and phenazines, for competition. We found that P. aeruginosa dominates cocultures with B. multivorans and that the three antimicrobials together promote P. aeruginosa competitiveness, with hydrogen cyanide contributing the greatest effect. We show that these QS-regulated antimicrobials are also critical for P. aeruginosa to prevent B. multivorans from cheating under nutrient conditions where both species require a P. aeruginosa quorum-regulated protease for growth. Together our results highlight the importance of antimicrobials in protecting cooperating populations from exploitation by other species that can act as cheaters.
    Description
    This is the published version. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
    URI
    http://hdl.handle.net/1808/18857
    DOI
    https://doi.org/10.1128/JB.00482-15
    Collections
    • Molecular Biosciences Scholarly Works [590]
    Citation
    Smalley, Nicole E., Dingding An, Matthew R. Parsek, Josephine R. Chandler, and Ajai A. Dandekar. "Quorum Sensing Protects Pseudomonas Aeruginosa against Cheating by Other Species in a Laboratory Coculture Model." Journal of Bacteriology J. Bacteriol. 197.19 (2015): 3154-159. http://dx.doi.org/10.1128/JB.00482-15.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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