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dc.contributor.authorSedlak, Ruth Hall
dc.contributor.authorHnilova, Marketa
dc.contributor.authorGrosh, Carolynn
dc.contributor.authorFong, Hanson
dc.contributor.authorBaneyx, Francois
dc.contributor.authorSchwartz, Dan
dc.contributor.authorSarikaya, Mehmet
dc.contributor.authorTamerler, Candan
dc.contributor.authorTraxler, Beth
dc.date.accessioned2015-10-29T20:43:38Z
dc.date.available2015-10-29T20:43:38Z
dc.date.issued2012-01-27
dc.identifier.citationSedlak, R. Hall, M. Hnilova, C. Grosh, H. Fong, F. Baneyx, D. Schwartz, M. Sarikaya, C. Tamerler, and B. Traxler. "Engineered Escherichia Coli Silver-Binding Periplasmic Protein That Promotes Silver Tolerance." Applied and Environmental Microbiology 78.7 (2012): 2289-296. http://dx.doi.org/10.1128/AEM.06823-11en_US
dc.identifier.urihttp://hdl.handle.net/1808/18755
dc.descriptionThis is the published version. Copyright © 2012, American Society for Microbiology. All Rights Reserved.en_US
dc.description.abstractSilver toxicity is a problem that microorganisms face in medical and environmental settings. Through exposure to silver compounds, some bacteria have adapted to growth in high concentrations of silver ions. Such adapted microbes may be dangerous as pathogens but, alternatively, could be potentially useful in nanomaterial-manufacturing applications. While naturally adapted isolates typically utilize efflux pumps to achieve metal resistance, we have engineered a silver-tolerant Escherichia coli strain by the use of a simple silver-binding peptide motif. A silver-binding peptide, AgBP2, was identified from a combinatorial display library and fused to the C terminus of the E. coli maltose-binding protein (MBP) to yield a silver-binding protein exhibiting nanomolar affinity for the metal. Growth experiments performed in the presence of silver nitrate showed that cells secreting MBP-AgBP2 into the periplasm exhibited silver tolerance in a batch culture, while those expressing a cytoplasmic version of the fusion protein or MBP alone did not. Transmission electron microscopy analysis of silver-tolerant cells revealed the presence of electron-dense silver nanoparticles. This is the first report of a specifically engineered metal-binding peptide exhibiting a strong in vivo phenotype, pointing toward a novel ability to manipulate bacterial interactions with heavy metals by the use of short and simple peptide motifs. Engineered metal-ion-tolerant microorganisms such as this E. coli strain could potentially be used in applications ranging from remediation to interrogation of biomolecule-metal interactions in vivo.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.titleEngineered Escherichia coli Silver-Binding Periplasmic Protein That Promotes Silver Toleranceen_US
dc.typeArticle
kusw.kuauthorTamerler, Candan
kusw.kudepartmentMechanical Engineeringen_US
dc.identifier.doi10.1128/AEM.06823-11
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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