KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Engineering
    • Mechanical Engineering Scholarly Works
    • View Item
    •   KU ScholarWorks
    • Engineering
    • Mechanical Engineering Scholarly Works
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Engineered Escherichia coli Silver-Binding Periplasmic Protein That Promotes Silver Tolerance

    Thumbnail
    View/Open
    Sedlak-escherichia_coli2012.pdf (1.113Mb)
    Issue Date
    2012-01-27
    Author
    Sedlak, Ruth Hall
    Hnilova, Marketa
    Grosh, Carolynn
    Fong, Hanson
    Baneyx, Francois
    Schwartz, Dan
    Sarikaya, Mehmet
    Tamerler, Candan
    Traxler, Beth
    Publisher
    American Society for Microbiology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
    Show full item record
    Abstract
    Silver toxicity is a problem that microorganisms face in medical and environmental settings. Through exposure to silver compounds, some bacteria have adapted to growth in high concentrations of silver ions. Such adapted microbes may be dangerous as pathogens but, alternatively, could be potentially useful in nanomaterial-manufacturing applications. While naturally adapted isolates typically utilize efflux pumps to achieve metal resistance, we have engineered a silver-tolerant Escherichia coli strain by the use of a simple silver-binding peptide motif. A silver-binding peptide, AgBP2, was identified from a combinatorial display library and fused to the C terminus of the E. coli maltose-binding protein (MBP) to yield a silver-binding protein exhibiting nanomolar affinity for the metal. Growth experiments performed in the presence of silver nitrate showed that cells secreting MBP-AgBP2 into the periplasm exhibited silver tolerance in a batch culture, while those expressing a cytoplasmic version of the fusion protein or MBP alone did not. Transmission electron microscopy analysis of silver-tolerant cells revealed the presence of electron-dense silver nanoparticles. This is the first report of a specifically engineered metal-binding peptide exhibiting a strong in vivo phenotype, pointing toward a novel ability to manipulate bacterial interactions with heavy metals by the use of short and simple peptide motifs. Engineered metal-ion-tolerant microorganisms such as this E. coli strain could potentially be used in applications ranging from remediation to interrogation of biomolecule-metal interactions in vivo.
    Description
    This is the published version. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
    URI
    http://hdl.handle.net/1808/18755
    DOI
    https://doi.org/10.1128/AEM.06823-11
    Collections
    • Bioengineering Program Scholarly Works [144]
    • Mechanical Engineering Scholarly Works [135]
    Citation
    Sedlak, R. Hall, M. Hnilova, C. Grosh, H. Fong, F. Baneyx, D. Schwartz, M. Sarikaya, C. Tamerler, and B. Traxler. "Engineered Escherichia Coli Silver-Binding Periplasmic Protein That Promotes Silver Tolerance." Applied and Environmental Microbiology 78.7 (2012): 2289-296. http://dx.doi.org/10.1128/AEM.06823-11

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps