dc.contributor.author | von Montfort, Claudia | |
dc.contributor.author | Sharov, Victor S. | |
dc.contributor.author | Metzger, Sabine | |
dc.contributor.author | Schoeneich, Christian | |
dc.contributor.author | Sies, Helmut | |
dc.contributor.author | Klotz, Lars-Oliver | |
dc.date.accessioned | 2015-05-08T18:41:15Z | |
dc.date.available | 2015-05-08T18:41:15Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Montfort et al. "Singlet oxygen inactivates protein tyrosine phosphatase-1B by oxidation of the active site cysteine." Biological Chemistry. Volume 387, Issue 10/11, Pages 1399–1404, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, November 2006.http://dx.doi.org/10.1515/BC.2006.175 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/17662 | |
dc.description.abstract | Singlet oxygen (1O2), an electronically excited form of molecular oxygen, is a mediator of biological effects of ultraviolet A radiation, stimulating signaling cascades in human cells. We demonstrate here that 1O2 generated by photosensitization or by thermodecomposition of 3,3′-(1,4-naphthylidene)dipropionate-1,4-endoperoxide inactivates isolated protein tyrosine phosphatases (PTPases). PTPase activities of PTP1B or CD45 were abolished by low concentrations of 1O2, but were largely restored by post-treatment with dithiothreitol. Electrospray ionization mass spectrometry analysis of tryptic digests of PTP1B exposed to 1O2 revealed oxidation of active-site Cys215 as the only cysteine residue oxidized. In summary, 1O2 may activate signaling cascades by interfering with phosphotyrosine dephosphorylation. | en_US |
dc.description.sponsorship | This study was supported by Deutsche Forschungsgemeinschaft (Bonn, Germany; Sonderforschungsbereich 503, Project B1). H.S. is a Fellow of the National Foundation for Cancer Research, Bethesda, MD, USA. | en_US |
dc.publisher | Walter de Gruyter | en_US |
dc.title | Singlet oxygen inactivates protein tyrosine phosphatase-1B by oxidation of the active site cysteine | en_US |
dc.type | Article | |
kusw.kuauthor | Sharov, Victor S. | |
kusw.kuauthor | Schoeneich, Christian | |
dc.identifier.doi | 10.1515/BC.2006.175 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |