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    Crystallographic characterization of the histidine protein kinase from an essential two-component regulatory system YycFG

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    TangLiang_ACF_64(4)346.pdf (240.8Kb)
    Issue Date
    2009-04-01
    Author
    Zhao, Haiyan
    Tang, Liang
    Publisher
    International Union of Crystallography
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    YycGF is a highly conserved two-component signal transduction system that is specific to low-G+C Gram-positive bacteria, including many important human pathogens. It has been recognized as a crucial regulatory system for cell-wall metabolism. YycG, the histidine protein kinase of this system, is a multidomain transmembrane protein. The truncated cytoplasmic portion of YycG from Bacillus subtilis encompassing the PAS domain, the dimerization domain and the catalytic domain was expressed, purified and crystallized. X-ray data were collected to 2.8 Å resolution with a completeness of 98.2% and an overall Rmerge of 5.6%. The crystals belonged to space group P61 or P65, with unit-cell parameters a = 135.0, c = 133.0 Å. The selenomethionine-substituted version of the protein was crystallized and X-ray data were collected to 3.6 Å resolution for subsequent MAD phasing.
    Description
    This is the publisher's version, also available electronically from "http://scripts.iucr.org".
    URI
    http://hdl.handle.net/1808/17600
    DOI
    https://doi.org/10.1107/S174430910900668X
    Collections
    • Molecular Biosciences Scholarly Works [598]
    Citation
    Zhao, H., & Tang, L. (2009). Crystallographic characterization of the histidine protein kinase from an essential two-component regulatory system YycFG. Acta Crystallografica F Struct Biol Cryst Commun., 64(4), 346-349. http://www.dx.doi.org/10.1107/S174430910900668X

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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