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dc.contributor.authorJohnson, Karyn N.
dc.contributor.authorTang, Liang
dc.contributor.authorJohnson, John E.
dc.contributor.authorBall, L. Andrew
dc.date.accessioned2015-05-05T20:03:10Z
dc.date.available2015-05-05T20:03:10Z
dc.date.issued2004-10-01
dc.identifier.citationJohnson, K., Tang, L., Johnson, J., & Ball, L. (2004). Heterologous RNA Encapsidated in Pariacoto Virus-Like Particles Forms a Dodecahedral Cage Similar to Genomic RNA in Wild-Type Virions. Journal of Virology, 78(20), 11371-11378. http://www.dx.doi.org/10.1128/JVI.78.20.11371-11378.2004en_US
dc.identifier.urihttp://hdl.handle.net/1808/17596
dc.descriptionThis is the publisher's version, also available electronically from "http://jvi.asm.org".en_US
dc.description.abstractThe genome of some icosahedral RNA viruses plays an essential role in capsid assembly and structure. In T=3 particles of the nodavirus Pariacoto virus (PaV), a remarkable 35% of the single-stranded RNA genome is icosahedrally ordered. This ordered RNA can be visualized at high resolution by X-ray crystallography as a dodecahedral cage consisting of 30 24-nucleotide A-form RNA duplex segments that each underlie a twofold icosahedral axis of the virus particle and interact extensively with the basic N-terminal region of 60 subunits of the capsid protein. To examine whether the PaV genome is a specific determinant of the RNA structure, we produced virus-like particles (VLPs) by expressing the wild-type capsid protein open reading frame from a recombinant baculovirus. VLPs produced by this system encapsidated similar total amounts of RNA as authentic virus particles, but only about 6% of this RNA was PaV specific, the rest being of cellular or baculovirus origin. Examination of the VLPs by electron cryomicroscopy and image reconstruction at 15.4-Å resolution showed that the encapsidated RNA formed a dodecahedral cage similar to that of wild-type particles. These results demonstrate that the specific nucleotide sequence of the PaV genome is not required to form the dodecahedral cage of ordered RNA.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.titleHeterologous RNA encapsidated in Pariacoto virus-like particles forms a dodecahedral cage similar to genomic RNA in wild-type virionsen_US
dc.typeArticle
kusw.kuauthorTang, Liang
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1128/JVI.78.20.11371-11378.2004
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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