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Heterologous RNA encapsidated in Pariacoto virus-like particles forms a dodecahedral cage similar to genomic RNA in wild-type virions
dc.contributor.author | Johnson, Karyn N. | |
dc.contributor.author | Tang, Liang | |
dc.contributor.author | Johnson, John E. | |
dc.contributor.author | Ball, L. Andrew | |
dc.date.accessioned | 2015-05-05T20:03:10Z | |
dc.date.available | 2015-05-05T20:03:10Z | |
dc.date.issued | 2004-10-01 | |
dc.identifier.citation | Johnson, K., Tang, L., Johnson, J., & Ball, L. (2004). Heterologous RNA Encapsidated in Pariacoto Virus-Like Particles Forms a Dodecahedral Cage Similar to Genomic RNA in Wild-Type Virions. Journal of Virology, 78(20), 11371-11378. http://www.dx.doi.org/10.1128/JVI.78.20.11371-11378.2004 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/17596 | |
dc.description | This is the publisher's version, also available electronically from "http://jvi.asm.org". | en_US |
dc.description.abstract | The genome of some icosahedral RNA viruses plays an essential role in capsid assembly and structure. In T=3 particles of the nodavirus Pariacoto virus (PaV), a remarkable 35% of the single-stranded RNA genome is icosahedrally ordered. This ordered RNA can be visualized at high resolution by X-ray crystallography as a dodecahedral cage consisting of 30 24-nucleotide A-form RNA duplex segments that each underlie a twofold icosahedral axis of the virus particle and interact extensively with the basic N-terminal region of 60 subunits of the capsid protein. To examine whether the PaV genome is a specific determinant of the RNA structure, we produced virus-like particles (VLPs) by expressing the wild-type capsid protein open reading frame from a recombinant baculovirus. VLPs produced by this system encapsidated similar total amounts of RNA as authentic virus particles, but only about 6% of this RNA was PaV specific, the rest being of cellular or baculovirus origin. Examination of the VLPs by electron cryomicroscopy and image reconstruction at 15.4-Å resolution showed that the encapsidated RNA formed a dodecahedral cage similar to that of wild-type particles. These results demonstrate that the specific nucleotide sequence of the PaV genome is not required to form the dodecahedral cage of ordered RNA. | en_US |
dc.publisher | American Society for Microbiology | en_US |
dc.title | Heterologous RNA encapsidated in Pariacoto virus-like particles forms a dodecahedral cage similar to genomic RNA in wild-type virions | en_US |
dc.type | Article | |
kusw.kuauthor | Tang, Liang | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1128/JVI.78.20.11371-11378.2004 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |