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    The role of protein kinase C in thromboxane A<sub>2</sub> - induced pulmonary artery vasoconstriciton

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    OrrJ_JoBS_6(4)293.pdf (291.0Kb)
    Issue Date
    1999-07-01
    Author
    Murtha, Yvonne M.
    Allen, Brandy M.
    Orr, James A.
    Publisher
    BioMed Central
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    In order to determine if protein kinase C (PKC) plays a significant role in the stimulant action of thromboxane A2 (TxA2) on pulmonary vascular smooth muscle, TxA2-induced contractile responses were measured following inhibition of PKC. Rabbits were sacrificed and segments of the main trunk of the pulmonary artery were removed and placed within a temperature-controlled (37°C) organ bath. Contractile responses that were evoked by a TxA2 mimetic (U46,619, 0.5 μM) decreased by 27 and 35% following treatment with the PKC inhibitors, calphostin C (2 μM) and staurosporine (200 nM), respectively. These results account for the effect of the vehicle, DMSO, which was also found to have a concentration-dependent inhibitory effect on the U46,619-induced contractions. The effects of DMSO alone was subsequently subtracted from the previously measured responses to PKC inhibitors that were dissolved in DMSO to obtain effects attributable to the PKC inhibitor alone. It can therefore be concluded that inhibition of PKC results in partial attenuation of U46,619-induced responses supporting the hypothesis that activation of PKC plays a partial role in TxA2-induced contraction of pulmonary arterial smooth muscle.
    Description
    This is the publisher's version, also available electronically from "http://www.karger.com".
    URI
    http://hdl.handle.net/1808/17560
    DOI
    https://doi.org/10.1159/000025398
    Collections
    • Molecular Biosciences Scholarly Works [537]
    Citation
    Murtha, Y., Allen, B., & Orr, J. (1999). The role of protein kinase C in thromboxane A2 - induced pulmonary artery vasoconstriciton. J. of Biomedical Sci., 6(4), 293-295. http://www.dx.doi.org/10.1159/000025398

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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