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    Spindle formation in Aspergillus is coupled to tubulin movement into the nucleus

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    Oakley_MBC_14(5)2192.pdf (238.4Kb)
    Issue Date
    2003-05-01
    Author
    Ovechkina, Yulia
    Maddox, Paul
    Oakley, C. Elizabeth
    Xiang, Xin
    Osmani, Stephen A.
    Salmon, Edward D.
    Oakley, Berl R.
    Publisher
    American Society for Cell Biology
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    In many important organisms, including many algae and most fungi, the nuclear envelope does not disassemble during mitosis. This fact raises the possibility that mitotic onset and/or exit might be regulated, in part, by movement of important mitotic proteins into and out of the nucleoplasm. We have used two methods to determine whether tubulin levels in the nucleoplasm are regulated in the fungus Aspergillus nidulans. First, we have used benomyl to disassemble microtubules and create a pool of free tubulin that can be readily observed by immunofluorescence. We find that tubulin is substantially excluded from interphase nuclei, but is present in mitotic nuclei. Second, we have observed a green fluorescent protein/α-tubulin fusion in living cells by time-lapse spinning-disk confocal microscopy. We find that tubulin is excluded from interphase nuclei, enters the nucleus seconds before the mitotic spindle begins to form, and is removed from the nucleoplasm during the M-to-G1 transition. Our data indicate that regulation of intranuclear tubulin levels plays an important, perhaps essential, role in the control of mitotic spindle formation in A. nidulans. They suggest that regulation of protein movement into the nucleoplasm may be important for regulating mitotic onset in organisms with intranuclear mitosis.
    Description
    This is the publisher's version, also available electronically from "http://www.molbiolcell.org".
    URI
    http://hdl.handle.net/1808/17508
    DOI
    https://doi.org/10.1091/mbc.E02-10-0641
    Collections
    • Molecular Biosciences Scholarly Works [590]
    Citation
    Ovechkina, Y., Maddox, P., Oakley, C.E., Xiang, X., Osmani, S.A., Salmon, E.D. & Oakley, B.R. (2003).Spindle formation in Aspergillus is coupled to tubulin movement into the nucleus. Molecular Biology of the Cell, 14(5), 2192-2200. http://www.dx.doi.org/10.1091/mbc.E02-10-0641

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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