dc.contributor.author | Prigozhina, Natalie L. | |
dc.contributor.author | Walker, Richard A. | |
dc.contributor.author | Oakley, C. Elizabeth | |
dc.contributor.author | Oakley, Berl R. | |
dc.date.accessioned | 2015-04-23T20:00:37Z | |
dc.date.available | 2015-04-23T20:00:37Z | |
dc.date.issued | 2001-10-01 | |
dc.identifier.citation | Prigozhina, N., Walker, R., Oakley, C., & Oakley, B. (2001). γ-Tubulin and the C-terminal motor domain kinesin-like protein, KLPA, function in the establishment of spindle bipolarity in Aspergillus nidulans. Molecular Biology of the Cell, 12(10), 3161-3174. http://www.dx.doi.org/10.1091/mbc.12.10.3161 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/17506 | |
dc.description | This is the publisher's version, also available electronically from "http://www.citationmachine.net". | en_US |
dc.description.abstract | Previous research has found that a γ-tubulin mutation inSchizosaccharomyces pombe is synthetically lethal with a deletion of the C-terminal motor domain kinesin-like protein genepkl1, but the lethality of the double mutant prevents a phenotypic analysis of the synthetic interaction. We have investigated interactions between klpA1, a deletion of an Aspergillus nidulans homolog of pkl1, and mutations in the mipA, γ-tubulin gene. We find that klpA1 dramatically increases the cold sensitivity and slightly reduces the growth rate at all temperatures, of threemipA alleles. In synchronized cells we find thatklpA1 causes a substantial but transient inhibition of the establishment of spindle bipolarity. At a restrictive temperature,mipAD123 causes a slight, transient inhibition of spindle bipolarity and a more significant inhibition of anaphase A. In the mipAD123/klpA1 strain, formation of bipolar spindles is more strongly inhibited than in theklpA1 single mutant and many spindles apparently never become bipolar. These results indicate, surprisingly, that γ-tubulin and the klpA kinesin have overlapping roles in the establishment of spindle bipolarity. We propose a model to account for these data. | en_US |
dc.publisher | American Society for Cell Biology | en_US |
dc.title | γ-Tubulin and the C-terminal motor domain kinesin-like protein, KLPA, function in the establishment of spindle bipolarity in Aspergillus nidulans | en_US |
dc.type | Article | |
kusw.kuauthor | Oakley, Berl R. | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1091/mbc.12.10.3161 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |