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dc.contributor.authorPrigozhina, Natalie L.
dc.contributor.authorWalker, Richard A.
dc.contributor.authorOakley, C. Elizabeth
dc.contributor.authorOakley, Berl R.
dc.date.accessioned2015-04-23T20:00:37Z
dc.date.available2015-04-23T20:00:37Z
dc.date.issued2001-10-01
dc.identifier.citationPrigozhina, N., Walker, R., Oakley, C., & Oakley, B. (2001). γ-Tubulin and the C-terminal motor domain kinesin-like protein, KLPA, function in the establishment of spindle bipolarity in Aspergillus nidulans. Molecular Biology of the Cell, 12(10), 3161-3174. http://www.dx.doi.org/10.1091/mbc.12.10.3161en_US
dc.identifier.urihttp://hdl.handle.net/1808/17506
dc.descriptionThis is the publisher's version, also available electronically from "http://www.citationmachine.net".en_US
dc.description.abstractPrevious research has found that a γ-tubulin mutation inSchizosaccharomyces pombe is synthetically lethal with a deletion of the C-terminal motor domain kinesin-like protein genepkl1, but the lethality of the double mutant prevents a phenotypic analysis of the synthetic interaction. We have investigated interactions between klpA1, a deletion of an Aspergillus nidulans homolog of pkl1, and mutations in the mipA, γ-tubulin gene. We find that klpA1 dramatically increases the cold sensitivity and slightly reduces the growth rate at all temperatures, of threemipA alleles. In synchronized cells we find thatklpA1 causes a substantial but transient inhibition of the establishment of spindle bipolarity. At a restrictive temperature,mipAD123 causes a slight, transient inhibition of spindle bipolarity and a more significant inhibition of anaphase A. In the mipAD123/klpA1 strain, formation of bipolar spindles is more strongly inhibited than in theklpA1 single mutant and many spindles apparently never become bipolar. These results indicate, surprisingly, that γ-tubulin and the klpA kinesin have overlapping roles in the establishment of spindle bipolarity. We propose a model to account for these data.en_US
dc.publisherAmerican Society for Cell Biologyen_US
dc.titleγ-Tubulin and the C-terminal motor domain kinesin-like protein, KLPA, function in the establishment of spindle bipolarity in Aspergillus nidulansen_US
dc.typeArticle
kusw.kuauthorOakley, Berl R.
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1091/mbc.12.10.3161
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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