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dc.contributor.authorJung, M. Katherine
dc.contributor.authorPrigozhina, Natalie L.
dc.contributor.authorOakley, C. Elizabeth
dc.contributor.authorNogales, Eva
dc.contributor.authorOakley, Berl R.
dc.date.accessioned2015-04-21T21:20:43Z
dc.date.available2015-04-21T21:20:43Z
dc.date.issued2001-07-01
dc.identifier.citationJung, M., Prigozhina, N., Oakley, C., Nogales, E., & Oakley, B. (2001). Alanine-scanning mutagenesis of Aspergillus γ-tubulin yields diverse and novel phenotypes. Moleculary Biology of the Cell, 12(7), 2119-2136. http://www.dx.doi.org/10.1091/mbc.12.7.2119en_US
dc.identifier.issn1059-1524
dc.identifier.urihttp://hdl.handle.net/1808/17462
dc.descriptionThis is the publisher's version, also available electronically from "www.molbiolcell.org".en_US
dc.description.abstractWe have created 41 clustered charged-to-alanine scanning mutations of the mipA, γ-tubulin, gene of Aspergillus nidulans and have created strains carrying these mutations by two-step gene replacement and by a new procedure, heterokaryon gene replacement. Most mutant alleles confer a wild-type phenotype, but others are lethal or conditionally lethal. The conditionally lethal alleles exhibit a variety of phenotypes under restrictive conditions. Most have robust but highly abnormal mitotic spindles and some have abnormal cytoplasmic microtubule arrays. Two alleles appear to have reduced amounts of γ-tubulin at the spindle pole bodies and nucleation of spindle microtubule assembly may be partially inhibited. One allele inhibits germ tube formation. The cold sensitivity of two alleles is strongly suppressed by the antimicrotubule agents benomyl and nocodazole and a third allele is essentially dependent on these compounds for growth. Together our data indicate that γ-tubulin probably carries out functions essential to mitosis and organization of cytoplasmic microtubules in addition to its well-documented role in microtubule nucleation. We have also placed our mutations on a model of the structure of γ-tubulin and these data give a good initial indication of the functionally important regions of the molecule.en_US
dc.publisherAmerican Society for Cell Biologyen_US
dc.titleAlanine-scanning mutagenesis of Aspergillus γ-tubulin yields diverse and novel phenotypesen_US
dc.typeArticle
kusw.kuauthorOakley, Berl R.
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1091/mbc.12.7.2119
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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