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dc.contributor.authorTam, Lai-Wa
dc.contributor.authorDentler, William L., Jr
dc.contributor.authorLefebvre, Paul A.
dc.identifier.citationTam, L.-W. "Defective Flagellar Assembly and Length Regulation in LF3 Null Mutants in Chlamydomonas." The Journal of Cell Biology 163.3 (2003): 597-607.
dc.description.abstractFour long-flagella (LF) genes are important for flagellar length control in Chlamydomonas reinhardtii. Here, we characterize two new null lf3 mutants whose phenotypes are different from previously identified lf3 mutants. These null mutants have unequal-length flagella that assemble more slowly than wild-type flagella, though their flagella can also reach abnormally long lengths. Prominent bulges are found at the distal ends of short, long, and regenerating flagella of these mutants. Analysis of the flagella by electron and immunofluorescence microscopy and by Western blots revealed that the bulges contain intraflagellar transport complexes, a defect reported previously (for review see Cole, D.G., 2003. Traffic. 4:435–442) in a subset of mutants defective in intraflagellar transport. We have cloned the wild-type LF3 gene and characterized a hypomorphic mutant allele of LF3. LF3p is a novel protein located predominantly in the cell body. It cosediments with the product of the LF1 gene in sucrose density gradients, indicating that these proteins may form a functional complex to regulate flagellar length and assembly.en_US
dc.publisherThe Rockefeller University Pressen_US
dc.rightsThe author reserves all rights under the creative commons license.
dc.titleDefective flagellar assembly and length regulation in LF3 null mutants in Chlamydomonasen_US
kusw.kuauthorDentler, William L., Jr
kusw.kudepartmentMolecular Biosciencesen_US
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.

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