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dc.contributor.authorPorter, Mary E.
dc.contributor.authorBower, Raqual
dc.contributor.authorKnott, Julie A.
dc.contributor.authorByrd, Pamela
dc.contributor.authorDentler, William L., Jr
dc.date.accessioned2015-03-09T21:26:49Z
dc.date.available2015-03-09T21:26:49Z
dc.date.issued1999-03-01
dc.identifier.citationPorter, Mary E. et al. (1999). "Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonas." Molecular Biology of the Cell, 10(3):693-712. http://www.dx.doi.org/10.1091/mbc.10.3.693.en_US
dc.identifier.issn1059-1524
dc.identifier.urihttp://hdl.handle.net/1808/17018
dc.description.abstractA second cytoplasmic dynein heavy chain (cDhc) has recently been identified in several organisms, and its expression pattern is consistent with a possible role in axoneme assembly. We have used a genetic approach to ask whether cDhc1b is involved in flagellar assembly in Chlamydomonas. Using a modified PCR protocol, we recovered two cDhc sequences distinct from the axonemal Dhc sequences identified previously. cDhc1a is closely related to the major cytoplasmic Dhc, whereas cDhc1b is closely related to the minor cDhc isoform identified in sea urchins, Caenorhabditis elegans, and Tetrahymena. TheChlamydomonas cDhc1b transcript is a low-abundance mRNA whose expression is enhanced by deflagellation. To determine its role in flagellar assembly, we screened a collection of stumpy flagellar (stf) mutants generated by insertional mutagenesis and identified two strains in which portions of the cDhc1bgene have been deleted. The two mutants assemble short flagellar stumps (<1–2 μm) filled with aberrant microtubules, raft-like particles, and other amorphous material. The results indicate that cDhc1b is involved in the transport of components required for flagellar assembly in Chlamydomonas.en_US
dc.publisherAmerican Society for Cell Biologyen_US
dc.rightsThis article is also available electronically from http://www.molbiolcell.org/content/10/3/693. This article was published under a Creative Commons Attribution Non-Commerical Share Alike 3.0 License.
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
dc.titleCytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonasen_US
dc.typeArticle
kusw.kuauthorByrd, Pamela
kusw.kuauthorDentler, William
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1091/mbc.10.3.693
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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This article is also available electronically from http://www.molbiolcell.org/content/10/3/693. This article was published under a Creative Commons Attribution Non-Commerical Share Alike 3.0 License.
Except where otherwise noted, this item's license is described as: This article is also available electronically from http://www.molbiolcell.org/content/10/3/693. This article was published under a Creative Commons Attribution Non-Commerical Share Alike 3.0 License.