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    Ciliary microtubule capping structures contain a mammalian kinetochore antigen

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    Dentler_March1-1990.pdf (3.569Mb)
    Issue Date
    1990-03-01
    Author
    Miller, J. M.
    Wang, W.
    Balczon, R.
    Dentler, William L., Jr
    Publisher
    Rockefeller University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    Structures that cap the plus ends of microtubules may be involved in the regulation of their assembly and disassembly. Growing and disassembling microtubules in the mitotic apparatus are capped by kinetochores and ciliary and flagellar microtubules are capped by the central microtubule cap and distal filaments. To compare the ciliary caps with kinetochores, isolated Tetrahymena cilia were stained with CREST (Calcinosis/phenomenon esophageal dysmotility, sclerodactyly, telangiectasia) antisera known to stain kinetochores. Immunofluorescence microscopy revealed that a CREST antiserum stained the distal tips of cilia that contained capping structures but did not stain axonemes that lacked capping structures. Both Coomassie blue-stained gels and Western blots probed with CREST antiserum revealed that a 97-kD antigen copurifies with the capping structures. Affinity-purified antibodies to the 97-kD ciliary protein stained the tips of cap-containing Tetrahymena cilia and the kinetochores in HeLa, Chinese hamster ovary, and Indian muntjak cells. These results suggest that at least one polypeptide found in the kinetochore is present in ciliary microtubule capping structures and that there may be a structural and/or functional homology between these structures that cap the plus ends of microtubules.
    Description
    This is the publisher's version, also available electronically from http://jcb.rupress.org/content/110/3/703.
    URI
    http://hdl.handle.net/1808/17016
    DOI
    https://doi.org/10.1083/jcb.110.3.703
    ISSN
    0021-9525
    Collections
    • Molecular Biosciences Scholarly Works [590]
    Citation
    Miller, J. M.; Wang, W.; Balczon, R.; Dentler, William L., Jr. (1990). "Ciliary microtubule capping structures contain a mammalian kinetochore antigen." Journal of Cell Biology, 110(3):703-714. http://www.dx.doi.org/10.1083/jcb.110.3.703.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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