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    Fractionation of Tetrahymena ciliary membranes with triton X-114 and the identification of a ciliary membrane ATPase

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    Dentler_Dec1-1988.pdf (2.254Mb)
    Issue Date
    1988-12-01
    Author
    Dentler, William L., Jr
    Publisher
    Rockefeller University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    Cilia were isolated from Tetrahymena thermophila, extracted with Triton X-114, and the detergent-soluble membrane + matrix proteins separated into Triton X-114 aqueous and detergent phases. The aqueous phase polypeptides include a high molecular mass polypeptide previously identified as a membrane dynein, detergent-soluble alpha and beta tubulins, and numerous polypeptides distinct from those found in axonemes. Integral membrane proteins partition into the detergent phase and include two major polypeptides of 58 and 50 kD, a 49-kD polypeptide, and 5 polypeptides in relatively minor amounts. The major detergent phase polypeptides are PAS-positive and are phosphorylated in vivo. A membrane-associated ATPase, distinct from the dynein-like protein, partitions into the Triton X-114 detergent phase and contains nearly 20% of the total ciliary ATPase activity. The ATPase requires Mg++ or Ca++ and is not inhibited by ouabain or vanadate. This procedure provides a gentle and rapid technique to separate integral membrane proteins from those that may be peripherally associated with the matrix or membrane.
    Description
    This is the publisher's version, also available electronically from http://jcb.rupress.org/content/107/6/2679.
    URI
    http://hdl.handle.net/1808/17011
    DOI
    https://doi.org/10.1083/jcb.107.6.2679
    ISSN
    0021-9525
    Collections
    • Molecular Biosciences Scholarly Works [598]
    Citation
    Dentler, William L., Jr (1988). "Fractionation of Tetrahymena ciliary membranes with triton X-114 and the identification of a ciliary membrane ATPase." Journal of Cell Biology, 107(6):2679-2688. http://www.dx.doi.org/10.1083/jcb.107.6.2679

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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