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    Microtubule-membrane interactions in cilia. II. Photochemical cross-linking of bridge structures and the identification of a membrane-associated dynein-like ATPase

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    Dentler_JCB_1980_II.pdf (2.237Mb)
    Issue Date
    1980-02-01
    Author
    Dentler, William L., Jr
    Pratt, M. M.
    Stephens, R. E.
    Publisher
    Rockefeller University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    Photochemical cross-linking of both Tetrahymena and Aequipecten ciliary membrane proteins with the lipophilic reagent 4,4'-dithiobisphenylazide links together a high molecular weight dynein-like ATPase, membrane tubulin, and at least two other proteins. Electron microscopy of detergent-extracted cilia reveals that the cross-linked complex remains attached to the outer-doublet microtubules by a microtubule-membrane bridge. Cleavage of the reagent's disulfide bond releases the bridge-membrane complex and the dynein-like membrane-associated ATPase. Electron microscopy was used to ensure that the dynein-like protein did not result from the solubilization of the dynein arms attached to the outer-doublet microtubules. The dynein-like protein has been isolated using sucrose gradients and is similar to axonemal dynein with respect to its sedimentation characteristics nucleotide specificity, and divalent cation requirements. Photochemical cross-linking of ciliary membrane porteins in vivo results initially in the modification of ciliary beat and, eventually, in the cessation of ciliary movement. These results suggest that a dynein-like ATPase comprises the bridge which links the ciliary membrane to the outer-doublet microtubules and that this bridge is involved in the modulation of normal ciliary movement.
    Description
    This is the publisher's version, also available electronically from http://jcb.rupress.org/content/84/2/381.
    URI
    http://hdl.handle.net/1808/17007
    DOI
    https://doi.org/10.1083/jcb.84.2.381
    ISSN
    0021-9525
    Collections
    • Molecular Biosciences Scholarly Works [606]
    Citation
    Dentler, William L., Jr; Pratt, M. M.; Stephens, R. E. (1980). "Microtubule-membrane interactions in cilia. II. Photochemical cross-linking of bridge structures and the identification of a membrane-associated dynein-like ATPase." Journal of Cell Biology, 84(2):381-403. http://www.dx.doi.org/10.1083/jcb.84.2.381.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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