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SUSP1 antagonizes formation of highly SUMO2/3-conjugated species
dc.contributor.author | Mukhopadhyay, Debaditya | |
dc.contributor.author | Ayaydin, Ferhan | |
dc.contributor.author | Kolli, Nagamalleswari | |
dc.contributor.author | Tan, Shyh-Han | |
dc.contributor.author | Anan, Tadashi | |
dc.contributor.author | Kametaka, Ai | |
dc.contributor.author | Azuma, Yoshiaki | |
dc.contributor.author | Wilkinson, Keith D. | |
dc.contributor.author | Dasso, Mary | |
dc.date.accessioned | 2014-10-07T18:31:35Z | |
dc.date.available | 2014-10-07T18:31:35Z | |
dc.date.issued | 2006-09-21 | |
dc.identifier.citation | Mukhopadhyay, Debaditya et al. (2006). "SUSP1 antagonizes formation of highly SUMO2/3-conjugated species." Journal of Cell Biology, 174(7):939-949. http://www.dx.doi.org/10.1083/jcb.200510103 | |
dc.identifier.issn | 0021-9525 | |
dc.identifier.uri | http://hdl.handle.net/1808/15208 | |
dc.description | This is the publisher's version, also available electronically from http://jcb.rupress.org/content/174/7/939 | |
dc.description.abstract | Small ubiquitin-related modifier (SUMO) processing and deconjugation are mediated by sentrin-specific proteases/ubiquitin-like proteases (SENP/Ulps). We show that SUMO-specific protease 1 (SUSP1), a mammalian SENP/Ulp, localizes within the nucleoplasm. SUSP1 depletion within cell lines expressing enhanced green fluorescent protein (EGFP) fusions to individual SUMO paralogues caused redistribution of EGFP-SUMO2 and -SUMO3, particularly into promyelocytic leukemia (PML) bodies. Further analysis suggested that this change resulted primarily from a deficit of SUMO2/3-deconjugation activity. Under these circumstances, PML bodies became enlarged and increased in number. We did not observe a comparable redistribution of EGFP-SUMO1. We have investigated the specificity of SUSP1 using vinyl sulfone inhibitors and model substrates. We found that SUSP1 has a strong paralogue bias toward SUMO2/3 and that it acts preferentially on substrates containing three or more SUMO2/3 moieties. Together, our findings argue that SUSP1 may play a specialized role in dismantling highly conjugated SUMO2 and -3 species that is critical for PML body maintenance. | |
dc.publisher | The Rockefeller University Press | |
dc.title | SUSP1 antagonizes formation of highly SUMO2/3-conjugated species | |
dc.type | Article | |
kusw.kuauthor | Azuma, Yoshiaki | |
kusw.kudepartment | Molecular Biosciences | |
kusw.oastatus | fullparticipation | |
dc.identifier.doi | 10.1083/jcb.200510103 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |