SUMO-2/3 regulates topoisomerase II in mitosis

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Issue Date
2003-11-03Author
Azuma, Yoshiaki
Arnaoutov, Alexei
Dasso, Mary
Publisher
The Rockefeller University Press
Type
Article
Article Version
Scholarly/refereed, publisher version
Metadata
Show full item recordAbstract
We have analyzed the abundance of SUMO-conjugated species during the cell cycle in Xenopus egg extracts. The predominant SUMO conjugation products associated with mitotic chromosomes arose from SUMO conjugation of topoisomerase II. Topoisomerase II was modified exclusively by SUMO-2/3 during mitosis under normal circumstances, although we observed conjugation of topoisomerase II to SUMO-1 in extracts with exogenous SUMO-1 protein. Inhibition of SUMO modification by a dominant-negative mutant of the SUMO-conjugating enzyme Ubc9 (dnUbc9) did not detectably alter topoisomerase II activity, but it did increase the amount of unmodified topoisomerase II retained on mitotic chromosomes after high salt washing. dnUbc9 did not disrupt the assembly of condensed mitotic chromosomes or block progression of extracts through mitosis, but it did block the dissociation of sister chromatids at the metaphase–anaphase transition. Together, our results suggest that SUMO conjugation is important for chromosome segregation in metazoan systems, and that mobilization of topoisomerase II from mitotic chromatin may be a key target of this modification.
Description
This is the publisher's version, also available electronically from http://jcb.rupress.org/content/163/3/477
ISSN
0021-9525Collections
Citation
Azuma, Yoshiaki, Arnaoutov, Alexei; Dasso, Mary. (2003). "SUMO-2/3 regulates topoisomerase II in mitosis." Journal of Cell Biology, 163(3):477-487. http://www.dx.doi.org/10.1083/jcb.200304088
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