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    SUMO-2/3 regulates topoisomerase II in mitosis

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    AzumaY_2003.pdf (398.2Kb)
    Issue Date
    2003-11-03
    Author
    Azuma, Yoshiaki
    Arnaoutov, Alexei
    Dasso, Mary
    Publisher
    The Rockefeller University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    We have analyzed the abundance of SUMO-conjugated species during the cell cycle in Xenopus egg extracts. The predominant SUMO conjugation products associated with mitotic chromosomes arose from SUMO conjugation of topoisomerase II. Topoisomerase II was modified exclusively by SUMO-2/3 during mitosis under normal circumstances, although we observed conjugation of topoisomerase II to SUMO-1 in extracts with exogenous SUMO-1 protein. Inhibition of SUMO modification by a dominant-negative mutant of the SUMO-conjugating enzyme Ubc9 (dnUbc9) did not detectably alter topoisomerase II activity, but it did increase the amount of unmodified topoisomerase II retained on mitotic chromosomes after high salt washing. dnUbc9 did not disrupt the assembly of condensed mitotic chromosomes or block progression of extracts through mitosis, but it did block the dissociation of sister chromatids at the metaphase–anaphase transition. Together, our results suggest that SUMO conjugation is important for chromosome segregation in metazoan systems, and that mobilization of topoisomerase II from mitotic chromatin may be a key target of this modification.
    Description
    This is the publisher's version, also available electronically from http://jcb.rupress.org/content/163/3/477
    URI
    http://hdl.handle.net/1808/15207
    DOI
    https://doi.org/10.1083/jcb.200304088
    ISSN
    0021-9525
    Collections
    • Molecular Biosciences Scholarly Works [598]
    Citation
    Azuma, Yoshiaki, Arnaoutov, Alexei; Dasso, Mary. (2003). "SUMO-2/3 regulates topoisomerase II in mitosis." Journal of Cell Biology, 163(3):477-487. http://www.dx.doi.org/10.1083/jcb.200304088

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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