Show simple item record

dc.contributor.authorBagby, Taryn Rochelle
dc.contributor.authorCai, Shuang
dc.contributor.authorDuan, Shaofeng
dc.contributor.authorThati, Sharadvi
dc.contributor.authorAires, Daniel J.
dc.contributor.authorForrest, M. Laird
dc.date.accessioned2014-04-14T14:10:48Z
dc.date.available2014-04-14T14:10:48Z
dc.date.issued2012-05-23
dc.identifier.citationBagby, Taryn R, Shuang Cai, Shaofeng Duan, Sharadvi Thati, Daniel J Aires, and Laird Forrest. 2012. “Impact of Molecular Weight on Lymphatic Drainage of a Biopolymer-Based Imaging Agent.” Pharmaceutics 4 (2): 276–95. http://dx.doi.org/10.3390/pharmaceutics4020276
dc.identifier.urihttp://hdl.handle.net/1808/13476
dc.description.abstractNew lymphatic imaging technologies are needed to better assess immune function and cancer progression and treatment. Lymphatic uptake depends mainly on particle size (10–100 nm) and charge. The size of carriers for imaging and drug delivery can be optimized to maximize lymphatic uptake, localize chemotherapy to lymphatic metastases, and enable visualization of treatment deposition. Toward this end, female BALB/c mice were injected subcutaneously in the hind footpad or forearm with a series of six different molecular weight hyaluronan (HA) near-infrared dye (HA-IR820) conjugates (ca. 5–200 nm). Mice were imaged using whole body fluorescent imaging over two weeks. HA-IR820 fluorescence was clearly visualized in the draining lymphatic capillaries, and in the popliteal and iliac or axillary lymph nodes. The 74-kDa HA-IR820 had the largest lymph node area-under-the-curve. In contrast to prior reports, mice bearing limb tumors exhibited three-fold longer retention of 74-kDa HA-IR820 in the popliteal node compared to mice without tumors. HA conjugate kinetics and disposition can be specifically tailored by altering their molecular weight. The specific lymphatic uptake and increased nodal retention of HA conjugates indicate significant potential for development as a natural biopolymer for intralymphatic drug delivery and imaging.
dc.publisherMDPI
dc.rightsThis article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectLymphatic imaging
dc.subjectHyaluronan
dc.subjectFluorescence
dc.subjectNanoparticle
dc.subjectTumor metastasis
dc.titleImpact of Molecular Weight on Lymphatic Drainage of a Biopolymer-Based Imaging Agent
dc.typeArticle
kusw.kuauthorBagby, Taryn Rochelle
kusw.kuauthorCai, Shuang
kusw.kuauthorDuan, Shaofeng
kusw.kuauthorThati, Sharadvi
kusw.kuauthorForrest, M. Laird
kusw.kudepartmentPharmaceutical Chemistry
dc.identifier.doi10.3390/pharmaceutics4020276
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Except where otherwise noted, this item's license is described as: This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).