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dc.contributor.authorHuang, Qinfeng
dc.contributor.authorDeng, Xuefeng
dc.contributor.authorYan, Ziying
dc.contributor.authorCheng, Fang
dc.contributor.authorLou, Yong
dc.contributor.authorShen, Weiran
dc.contributor.authorLei-Butters, Diana C. M.
dc.contributor.authorChen, Aaron Yun
dc.contributor.authorLi, Yi
dc.contributor.authorTang, Liang
dc.contributor.authorSöderlund-Venermo, Maria
dc.contributor.authorEnglehardt, John F.
dc.contributor.authorQiu, Jianming
dc.date.accessioned2014-03-19T15:10:09Z
dc.date.available2014-03-19T15:10:09Z
dc.date.issued2012-08-30
dc.identifier.citationHuang, Q., Deng, X., Yan, Z., Cheng, F., Luo, Y., Shen, W., … Qiu, J. (2012). Establishment of a Reverse Genetics System for Studying Human Bocavirus in Human Airway Epithelia. PLoS Pathog, 8(8). http://dx.doi.org/10.1371/journal.ppat.1002899
dc.identifier.urihttp://hdl.handle.net/1808/13249
dc.description.abstractHuman bocavirus 1 (HBoV1) has been identified as one of the etiological agents of wheezing in young children with acute respiratory-tract infections. In this study, we have obtained the sequence of a full-length HBoV1 genome (including both termini) using viral DNA extracted from a nasopharyngeal aspirate of an infected patient, cloned the full-length HBoV1 genome, and demonstrated DNA replication, encapsidation of the ssDNA genome, and release of the HBoV1 virions from human embryonic kidney 293 cells. The HBoV1 virions generated from this cell line-based production system exhibits a typical icosahedral structure of approximately 26 nm in diameter, and is capable of productively infecting polarized primary human airway epithelia (HAE) from the apical surface. Infected HAE showed hallmarks of lung airway-tract injury, including disruption of the tight junction barrier, loss of cilia and epithelial cell hypertrophy. Notably, polarized HAE cultured from an immortalized airway epithelial cell line, CuFi-8 (originally derived from a cystic fibrosis patient), also supported productive infection of HBoV1. Thus, we have established a reverse genetics system and generated the first cell line-based culture system for the study of HBoV1 infection, which will significantly advance the study of HBoV1 replication and pathogenesis.
dc.description.sponsorshipThis work was supported by PHS R21 grant AI085236 and PHS R01 grant AI070723 from NIAID (J Qiu) and PHS R01 grant HL108902 from NHLBI (J Engelhardt).
dc.publisherPublic Library of Science
dc.rights©Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnitbodies
dc.subjectCell polarity
dc.subjectDNA replication
dc.subjectDNA sequences
dc.subjectNucleotide sequencing
dc.subjectRepiratory infections
dc.subjectViral replication
dc.subjectVirions
dc.titleEstablishment of a Reverse Genetics System for Studying Human Bocavirus in Human Airway Epithelia
dc.typeArticle
kusw.kuauthorTang, liang
kusw.kudepartmentMolecular Biosciences
kusw.oastatusfullparticipation
dc.identifier.doi10.1371/journal.ppat.1002899
dc.rights.holderopenAccess
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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©Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as: ©Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.