RACK-1 Acts with Rac GTPase Signaling and UNC-115/abLIM in Caenorhabditis elegans Axon Pathfinding and Cell Migration
dc.contributor.author | Demarco, Rafael Senos | |
dc.contributor.author | Lundquist, Erik A. | |
dc.date.accessioned | 2014-03-18T16:31:28Z | |
dc.date.available | 2014-03-18T16:31:28Z | |
dc.date.issued | 2010-11-18 | |
dc.identifier.citation | Demarco, R. S., & Lundquist, E. A. (2010). RACK-1 Acts with Rac GTPase Signaling and UNC-115/abLIM in Caenorhabditis elegans Axon Pathfinding and Cell Migration. PLoS Genet, 6(11). http://dx.doi.org/10.1371/journal.pgen.1001215 | |
dc.identifier.uri | http://hdl.handle.net/1808/13226 | |
dc.description.abstract | Migrating cells and growth cones extend lamellipodial and filopodial protrusions that are required for outgrowth and guidance. The mechanisms of cytoskeletal regulation that underlie cell and growth cone migration are of much interest to developmental biologists. Previous studies have shown that the Arp2/3 complex and UNC-115/abLIM act redundantly to mediate growth cone lamellipodia and filopodia formation and axon pathfinding. While much is known about the regulation of Arp2/3, less is known about regulators of UNC-115/abLIM. Here we show that the Caenorhabditis elegans counterpart of the Receptor for Activated C Kinase (RACK-1) interacts physically with the actin-binding protein UNC-115/abLIM and that RACK-1 is required for axon pathfinding. Genetic interactions indicate that RACK-1 acts cell-autonomously in the UNC-115/abLIM pathway in axon pathfinding and lamellipodia and filopodia formation, downstream of the CED-10/Rac GTPase and in parallel to MIG-2/RhoG. Furthermore, we show that RACK-1 is involved in migration of the gonadal distal tip cells and that the signaling pathways involved in this process might be distinct from those involved in axon pathfinding. In sum, these studies pinpoint RACK-1 as a component of a novel signaling pathway involving Rac GTPases and UNC-115/abLIM and suggest that RACK-1 might be involved in the regulation of the actin cytoskeleton and lamellipodia and filopodia formation in migrating cells and growth cones. | |
dc.description.sponsorship | This work was supported by NIH R01 NS040945 (http://www.nih.gov) and NSF IOB 93192 (http://www.nsf.gov) to EAL and NIH P20 RR16475 (PI: JS Hunt). Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the NIH National Center for Research Resources (NCRR). | |
dc.publisher | Public Library of Science | |
dc.rights | ©2010 Demarco, Lundquist. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Axon guidance | |
dc.subject | Axons | |
dc.subject | Caenorhabditis elegans | |
dc.subject | Cell migration | |
dc.subject | Complementary DNA | |
dc.subject | GTPase signaling | |
dc.subject | Neurons | |
dc.subject | Two-hybrids screening | |
dc.title | RACK-1 Acts with Rac GTPase Signaling and UNC-115/abLIM in Caenorhabditis elegans Axon Pathfinding and Cell Migration | |
dc.type | Article | |
kusw.kuauthor | Demarco, Rafael S. | |
kusw.kuauthor | Lundquist, Erik A. | |
kusw.kudepartment | Molecular Biosciences | |
kusw.oastatus | fullparticipation | |
dc.identifier.doi | 10.1371/journal.pgen.1001215 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: ©2010 Demarco, Lundquist. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.