PIASγ Is Required for Faithful Chromosome Segregation in Human Cells
dc.contributor.author | Díaz-Martínez, Laura A. | |
dc.contributor.author | Giménez-Abián, Juan F. | |
dc.contributor.author | Azuma, Yoshiaki | |
dc.contributor.author | Guacci, Vincent | |
dc.contributor.author | Giménez-Martín, Gonzalo | |
dc.contributor.author | Lanier, Lorene M. | |
dc.contributor.author | Clarke, Duncan J. | |
dc.date.accessioned | 2014-03-18T15:11:17Z | |
dc.date.available | 2014-03-18T15:11:17Z | |
dc.date.issued | 2006-12-20 | |
dc.identifier.citation | Díaz-Martínez, L. A., Giménez-Abián, J. F., Azuma, Y., Guacci, V., Giménez-Martín, G., Lanier, L. M., & Clarke, D. J. (2006). PIASγ Is Required for Faithful Chromosome Segregation in Human Cells. PLoS ONE, 1(1). http://dx.doi.org/10.1371/journal.pone.0000053 | |
dc.identifier.uri | http://hdl.handle.net/1808/13189 | |
dc.description.abstract | Background The precision of the metaphase-anaphase transition ensures stable genetic inheritance. The spindle checkpoint blocks anaphase onset until the last chromosome biorients at metaphase plate, then the bonds between sister chromatids are removed and disjoined chromatids segregate to the spindle poles. But, how sister separation is triggered is not fully understood. Principal Findings We identify PIASγ as a human E3 sumo ligase required for timely and efficient sister chromatid separation. In cells lacking PIASγ, normal metaphase plates form, but the spindle checkpoint is activated, leading to a prolonged metaphase block. Sister chromatids remain cohered even if cohesin is removed by depletion of hSgo1, because DNA catenations persist at centromeres. PIASγ-depleted cells cannot properly localize Topoisomerase II at centromeres or in the cores of mitotic chromosomes, providing a functional link between PIASγ and Topoisomerase II. Conclusions PIASγ directs Topoisomerase II to specific chromosome regions that require efficient removal of DNA catenations prior to anaphase. The lack of this activity activates the spindle checkpoint, protecting cells from non-disjunction. Because DNA catenations persist without PIASγ in the absence of cohesin, removal of catenations and cohesin rings must be regulated in parallel. | |
dc.description.sponsorship | This work was funded by NIH grant CA099033 (DJC). JFGA was partly supported by MEC-Spain DGI and LADM by CONACyT. | |
dc.publisher | Public Library of Science | |
dc.rights | ©2006 Diaz-Martinez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Anaphase | |
dc.subject | Cell staining | |
dc.subject | Centromeres | |
dc.subject | Chromatids | |
dc.subject | Chromosomes | |
dc.subject | HeLa cells | |
dc.subject | Metaphaseq | |
dc.subject | Mitosis | |
dc.title | PIASγ Is Required for Faithful Chromosome Segregation in Human Cells | |
dc.type | Article | |
kusw.kuauthor | Azuma, Yoshiaki | |
kusw.kudepartment | Molecular Biosciences | |
kusw.oastatus | na | |
dc.identifier.doi | 10.1371/journal.pone.0000053 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: ©2006 Diaz-Martinez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.