Show simple item record

dc.contributor.authorBeckert, Ulrike
dc.contributor.authorYih Aw, Wen
dc.contributor.authorBurhenne, Heike
dc.contributor.authorFörsterling, Lisa
dc.contributor.authorKaever, Volkhard
dc.contributor.authorTimmons, Lisa
dc.contributor.authorSeifert, Roland
dc.date.accessioned2014-03-17T20:12:02Z
dc.date.available2014-03-17T20:12:02Z
dc.date.issued2013-08-27
dc.identifier.citationBeckert U, Aw WY, Burhenne H, Försterling L, Kaever V, et al. (2013) The Receptor-Bound Guanylyl Cyclase DAF-11 Is the Mediator of Hydrogen Peroxide-Induced Cgmp Increase in Caenorhabditis elegans. PLoS ONE 8(8): e72569. http://dx.doi.org/10.1371/journal.pone.0072569
dc.identifier.urihttp://hdl.handle.net/1808/13181
dc.description.abstractAdenosine 3′, 5′-cyclic monophosphate (cAMP) and guanosine 3′, 5′-cyclic monophosphate (cGMP) are well-studied second messengers that transmit extracellular signals into mammalian cells, with conserved functions in various other species such as Caenorhabditis elegans (C. elegans). cAMP is generated by adenylyl cyclases, and cGMP is generated by guanylyl cyclases, respectively. Studies using C. elegans have revealed additional roles for cGMP signaling in lifespan extension. For example, mutants lacking the function of a specific receptor-bound guanylyl cyclase, DAF-11, have an increased life expectancy. While the daf-11 phenotype has been attributed to reductions in intracellular cGMP concentrations, the actual content of cyclic nucleotides has not been biochemically determined in this system. Similar assumptions were made in studies using phosphodiesterase loss-of-function mutants or using adenylyl cyclase overexpressing mutants. In the present study, cyclic nucleotide regulation in C. elegans was studied by establishing a special nematode protocol for the simultaneous detection and quantitation of cyclic nucleotides. We also examined the influence of reactive oxygen species (ROS) on cyclic nucleotide metabolism and lifespan in C. elegans using highly specific HPLC-coupled tandem mass-spectrometry and behavioral assays. Here, we show that the relation between cGMP and survival is more complex than previously appreciated.
dc.description.sponsorshipThis work was supported by a Fulbright graduate student fellowship to Ulrike Beckert and a German Academic Exchange Service RISE fellowship to Wen Yih Aw.
dc.publisherPublic Library of Science
dc.rights©2013 Beckert et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnalysis of variance
dc.subjectCaenorhabditis elegans
dc.subjectGene expression
dc.subjectLiquid chromatography-mass spectrometry
dc.subjectOxidative stress
dc.subjectPartial differential equations
dc.subjectPhosphodiesterases
dc.subjectcGMP signaling
dc.titleThe Receptor-Bound Guanylyl Cyclase DAF-11 Is the Mediator of Hydrogen Peroxide-Induced Cgmp Increase in Caenorhabditis elegans
dc.typeArticle
kusw.kuauthorAw, Wen Yih
kusw.kuauthorTimmons, Lisa
kusw.kudepartmentDepartment of Molecular Biosciences
kusw.oanotesPLOS project: This item has a Creative Commons license that allows it to be shared in KU ScholarWorks.
kusw.oastatusfullparticipation
dc.identifier.doi10.1371/journal.pone.0072569
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

©2013 Beckert et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as: ©2013 Beckert et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.