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dc.contributor.authorLu, Jiamiao
dc.contributor.authorDentler, William L., Jr
dc.contributor.authorLundquist, Erik A.
dc.date.accessioned2014-01-17T21:20:50Z
dc.date.available2014-01-17T21:20:50Z
dc.date.issued2008-05-16
dc.identifier.citationLu, Jiamiao, William L Dentler, and Erik A Lundquist. 2008. “FLI-1 Flightless-1 and LET-60 Ras Control Germ Line Morphogenesis in C. Elegans.” BMC Developmental Biology 8:54. http://dx.doi.org/10.1186/1471-213X-8-54.
dc.identifier.urihttp://hdl.handle.net/1808/12817
dc.description.abstractBackground: In the C. elegans germ line, syncytial germ line nuclei are arranged at the cortex of the germ line as they exit mitosis and enter meiosis, forming a nucleus-free core of germ line cytoplasm called the rachis. Molecular mechanisms of rachis formation and germ line organization are not well understood. Results: Mutations in the fli-1 gene disrupt rachis organization without affecting meiotic differentiation, a phenotype in C. elegans referred to here as the g erm l ine m orphogenesis (Glm) phenotype. In fli-1 mutants, chains of meiotic germ nuclei spanned the rachis and were partially enveloped by invaginations of germ line plasma membrane, similar to nuclei at the cortex. Extensions of the somatic sheath cells that surround the germ line protruded deep inside the rachis and were associated with displaced nuclei in fli-1 mutants. fli-1 encodes a molecule with leucine-rich repeats and gelsolin repeats similar to Drosophila flightless 1 and human Fliih, which have been shown to act as cytoplasmic actin regulators as well as nuclear transcriptional regulators. Mutations in let-60 Ras, previously implicated in germ line development, were found to cause the Glm phenotype. Constitutively-active LET-60 partially rescued the fli-1 Glm phenotype, suggesting that LET-60 Ras and FLI-1 might act together to control germ line morphogenesis. Conclusion: FLI-1 controls germ line morphogenesis and rachis organization, a process about which little is known at the molecular level. The LET-60 Ras GTPase might act with FLI-1 to control germ line morphogenesis.
dc.publisherBioMed Central
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.titleFLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in C. elegans
dc.typeArticle
kusw.kuauthorLu, Jiamiao
kusw.kuauthorDentler, William L.
kusw.kuauthorLundquist, Erik A.
kusw.kudepartmentMolecular Biosciences
kusw.oastatusfullparticipation
dc.identifier.doi10.1186/1471-213X-8-54
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.