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dc.contributor.authorOxana Norkinaen_US
dc.contributor.authorRolf Grafen_US
dc.contributor.authorPhilippe Appenzelleren_US
dc.contributor.authorRobert De Lisleen_US
dc.date.accessioned2009-05-05T16:43:53Z
dc.date.available2009-05-05T16:43:53Z
dc.date.issued2008-08-26en_US
dc.identifier.citationOxana Norkina;Rolf Graf;Philippe Appenzeller;Robert De Lisle: Caerulein-induced acute pancreatitis in mice that constitutively overexpress Reg/PAP genes. BMC Gastroenterol 2006, 6(1):16.en_US
dc.identifier.urihttp://hdl.handle.net/2271/632en_US
dc.description.abstractBACKGROUND:The cystic fibrosis (CF) mouse pancreas has constitutively elevated expression of the Reg/PAP cell stress genes (60-fold greater Reg3a, and 10-fold greater PAP/Reg3ß and Reg3?). These genes are suggested to be involved in protection or recovery from pancreatic injury.METHODS:To test this idea the supramaximal caerulein model was used to induce acute pancreatitis in wild type and CF mice. Serum amylase, pancreatic water content (as a measure of edema), pancreatic myeloperoxidase activity, and Reg/PAP expression were quantified.RESULTS:In both wild type and CF mice caerulein induced similar elevations in serum amylase (maximal at 12 h), pancreatic edema (maximal at 7 h), and pancreatic myeloperoxidase activity (MPO, a marker of neutrophil infiltration; maximal at 7 h). By immunohistochemistry, Reg3a was strongly expressed in the untreated CF pancreas but not in wild type. During pancreatitis, Reg3a was intensely expressed in foci of inflamed tissue in both wild type and CF.CONCLUSION:These data demonstrate that the severity of caerulein-induced pancreatitis is not ameliorated in the CF mouse even though the Reg/PAP stress genes are already highly upregulated. While Reg/PAP may be protective they may also have a negative effect during pancreatitis due to their anti-apoptotic activity, which has been shown to increase the severity of pancreatitis.en_US
dc.languageenen_US
dc.language.isoen_USen_US
dc.publisherBioMedCentralen_US
dc.relation.isversionofhttp://www.biomedcentral.com/1471-230X/6/16en_US
dc.relation.hasversionhttp://www.biomedcentral.com/content/pdf/1471-230X-6-16.pdfen_US
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAttitude of Health Personnelen_US
dc.subject.meshDecision Makingen_US
dc.subject.meshFactor Analysis, Statisticalen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshHypersensitivity/ diagnosisen_US
dc.subject.meshImmunoglobulin E/ blooden_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPhysician's Practice Patterns/statistics & numerical dataen_US
dc.subject.meshPhysicians, Family/psychology/ statistics & numerical dataen_US
dc.subject.meshQuestionnairesen_US
dc.subject.meshReferral and Consultationen_US
dc.subject.meshRegression Analysisen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshSoutheastern United Statesen_US
dc.titleCaerulein-induced acute pancreatitis in mice that constitutively overexpress Reg/PAP genesen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/1471-230X-6-16en_US
dc.identifier.pmid18727827en_US
dc.rights.accessrightsopenAccessen_US
dc.date.captured2009-04-27en_US


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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.