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dc.contributor.authorLea Rempelen_US
dc.contributor.authorKathleen Austinen_US
dc.contributor.authorKenneth Ritchieen_US
dc.contributor.authorMing Yanen_US
dc.contributor.authorMeifeng Shenen_US
dc.contributor.authorDong-Er Zhangen_US
dc.contributor.authorLuiz Henkesen_US
dc.contributor.authorThomas Hansenen_US
dc.date.accessioned2009-05-05T16:14:58Z
dc.date.available2009-05-05T16:14:58Z
dc.date.issued2006-10-09en_US
dc.identifier.citationLea Rempel;Kathleen Austin;Kenneth Ritchie;Ming Yan;Meifeng Shen;Dong-Er Zhang;Luiz Henkes;Thomas Hansen: Ubp43 gene expression is required for normal Isg15 expression and fetal development. Reproductive Biology and Endocrinology 2007, 5(1):13.en_US
dc.identifier.urihttp://hdl.handle.net/2271/603en_US
dc.description.abstractBACKGROUND:Isg15 covalently modifies murine endometrial proteins in response to early pregnancy. Isg15 can also be severed from targeted proteins by a specific protease called Ubp43 (Usp18). Mice lacking Ubp43 (null) form increased conjugated Isg15 in response to interferon. The Isg15 system has not been examined in chorioallantoic placenta (CP) or mesometrial (MM) components of implantation sites beyond 9.5 days post coitum (dpc). It was hypothesized that deletion of Ubp43 would cause disregulation of Isg15 in implantation sites, and that this would affect pregnancy rates.METHODS:Heterozygous (het) Ubp43 mice were mated and MM and CP implantation sites were collected on 12.5 and 17.5 days post-coitum (dpc).RESULTS:Free and conjugated Isg15 were greater on 12.5 versus 17.5 dpc in MM. Free and conjugated Isg15 were also present in CP, but did not differ due to genotype on 12.5 dpc. However, null CP had greater free and conjugated Isg15 when compared to het/wt on 17.5 dpc. Null progeny died in utero with fetal genotype ratios (wt:het:null) of 2:5:1 on 12.5 and 2:2:1 on 17.5 dpc. Implantation sites were disrupted within the junctional zone and spongiotrophoblast, contained less vasculature based on lectin B4 staining and contained greater Isg15 mRNA and VEGF protein in Ubp43 null when compared to wt placenta.CONCLUSION:It is concluded that Isg15 and its conjugates are present in implantation sites during mid to late gestation and that deletion of Ubp43 causes an increase in free and conjugated Isg15 at the feto-maternal interface. Also, under mixed genetic background, deletion of Ubp43 results in fetal death.en_US
dc.languageenen_US
dc.language.isoen_USen_US
dc.publisherBioMedCentralen_US
dc.relation.isversionofhttp://www.rbej.com/content/5/1/13en_US
dc.relation.hasversionhttp://www.biomedcentral.com/content/pdf/1477-7827-5-13.pdfen_US
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_US
dc.subject.meshAnimalsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation/drug effectsen_US
dc.subject.meshHLA Antigens/genetics/ physiologyen_US
dc.subject.meshHistocompatibility Antigens Class I/genetics/ physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunosuppressionen_US
dc.subject.meshMaternal-Fetal Exchange/genetics/immunology/physiologyen_US
dc.subject.meshOxygen/pharmacologyen_US
dc.subject.meshPapio/geneticsen_US
dc.subject.meshPlacenta/metabolism/physiologyen_US
dc.subject.meshPregnancyen_US
dc.subject.meshRegulatory Sequences, Nucleic Aciden_US
dc.titleUbp43 gene expression is required for normal Isg15 expression and fetal developmenten_US
dc.typeArticleen_US
dc.identifier.doi10.1186/1477-7827-5-13en_US
dc.identifier.pmid17118165en_US
dc.rights.accessrightsopenAccessen_US
dc.date.captured2009-04-27en_US


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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.