Induction of calcification by serum depletion in cell culture: a model for focal calcification in aortas related to atherosclerosis
dc.contributor.author | Howard Hsu | en_US |
dc.contributor.author | Antonio Artigues | en_US |
dc.contributor.author | Maria Villar | en_US |
dc.date.accessioned | 2009-05-05T16:14:39Z | |
dc.date.available | 2009-05-05T16:14:39Z | |
dc.date.issued | 2006-10-16 | en_US |
dc.identifier.citation | Howard Hsu;Antonio Artigues;Maria Villar: Induction of calcification by serum depletion in cell culture: a model for focal calcification in aortas related to atherosclerosis. Lipids Health Dis 2008, 7(1):2. | en_US |
dc.identifier.uri | http://hdl.handle.net/2271/600 | en_US |
dc.description.abstract | BACKGROUND:Since aortic calcification has been shown to initiate in the lower zone of well-thickened plaques (LZP) adjacent to the aortic media of rabbits fed supplemental cholesterol diets, a restricted supply of serum to vascular cells could play a role in vascular calcification. This study was designed to use a cell culture model to support this hypothesis.RESULTS:Rabbit aortic smooth muscle cells were grown to confluence in a culture media containing 10 % fetal bovine serum (FBS). The confluent cells were then exposed to the media for 2 hrs with or without serum at a Ca × P ion product range of 4.5 9.4 mM2. In contrast to the cells cultured in the presence of FBS, confluent cells in its absence displayed marked mineral-positive alizarin red staining and infrared absorption of mineral phosphate. A kinetic parameter C1/2 was used to designate the concentration of serum or its protein constituents needed to reduce the deposition of Ca and P by half. The C1/2 for FBS and rabbit serum was 0.04 0.07 % The C1/2 value for rabbit serum proteins was 13.5 µg/ml corresponding to the protein concentration in 0.06 % of serum. This C1/2 was markedly smaller than 86.2 µg/ml for bovine serum albumin present in 0.37 % serum (p < 0.05). Serum depletion also caused marked membrane translocation as evidenced through a specific apoptosis dye uptake by cells. The proteomic analysis of calcifying vesicles, which can be released by serum depletion, revealed several calcification-related proteins.CONCLUSION:The aortic smooth muscle cell culture model suggests that serum depletion may play a role in the initiation of aortic calcification. The serum exhibits remarkable ability to inhibit cell-mediated calcification. | en_US |
dc.language | en | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BioMedCentral | en_US |
dc.relation.isversionof | http://www.lipidworld.com/content/7/1/2 | en_US |
dc.relation.hasversion | http://www.biomedcentral.com/content/pdf/1476-511X-7-2.pdf | en_US |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0 | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Aorta, Thoracic/metabolism/ pathology | en_US |
dc.subject.mesh | Aortic Diseases/etiology/metabolism/pathology | en_US |
dc.subject.mesh | Biological Markers/metabolism | en_US |
dc.subject.mesh | Calcinosis/metabolism/ pathology | en_US |
dc.subject.mesh | Cholesterol, Dietary/adverse effects/ metabolism | en_US |
dc.subject.mesh | Cholesterol, LDL/ metabolism | en_US |
dc.subject.mesh | Coronary Artery Disease/etiology/metabolism/pathology | en_US |
dc.subject.mesh | Cytoplasmic Vesicles/metabolism/ pathology | en_US |
dc.subject.mesh | Diet, Atherogenic | en_US |
dc.subject.mesh | Dietary Supplements/adverse effects | en_US |
dc.subject.mesh | Hypercholesterolemia/blood | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Microsomes/metabolism/pathology | en_US |
dc.subject.mesh | Rabbits | en_US |
dc.subject.mesh | Tunica Intima/metabolism/ pathology | en_US |
dc.title | Induction of calcification by serum depletion in cell culture: a model for focal calcification in aortas related to atherosclerosis | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1186/1476-511X-7-2 | en_US |
dc.identifier.pmid | PMC17042957 | en_US |
dc.rights.accessrights | openAccess | en_US |
dc.date.captured | 2009-04-27 | en_US |
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Except where otherwise noted, this item's license is described as: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.