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dc.contributor.advisorNishimune, Hiroshien_US
dc.contributor.authorBillings, Sara Elizabeth
dc.date.accessioned2012-06-03T14:11:10Z
dc.date.available2012-06-03T14:11:10Z
dc.date.issued2011-12-31en_US
dc.date.submitted2011en_US
dc.identifier.otherhttp://dissertations.umi.com/ku:11840en_US
dc.identifier.urihttp://hdl.handle.net/1808/9725en_US
dc.description.abstractThe cytoskeletal matrix of the active zone (CAZ) and synaptic voltage-dependent calcium channels (VDCCs) are both required for the organization and regulation of synaptic vesicle release. Here we report a novel interaction between the CAZ protein family ELKS and VDCC β subunits. We found that two isoforms of ELKS (ELKS2 and ELKS1b) and two isoforms of VDCC β subunits (β1b and β4) co-immunoprecipitated. Using fluorescent immunohistochemistry, we observed colocalization between ELKS1b and VDCC β4 at synapses in the molecular layer of the cerebellum, suggesting a synaptic role for the interaction of these two proteins. Analysis of a P/Q-type VDCC knockout mouse (Cacna1a-/-) revealed that the localization of the VDCC β4 subunit to the molecular layer was disrupted, although ELKS1b protein localization was not affected. The results demonstrate that two isoforms of the ELKS family and two VDCC β subunits interact and suggest that their interaction may play a role in the organization of the active zone.
dc.format.extent48 pagesen_US
dc.language.isoen_USen_US
dc.publisherUniversity of Kansasen_US
dc.rightsThis item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.en_US
dc.subjectNeurosciences
dc.subjectCell biology
dc.subjectBiology
dc.subjectActive zone
dc.subjectCast
dc.subjectCytomatrix of the active zone
dc.subjectElks
dc.subjectSynapse
dc.subjectVoltage-dependent calcium channel
dc.titleThe interaction of ELKS with Ca2+ channel β subunits may contribute to active zone organization
dc.typeThesisen_US
dc.contributor.cmtememberAckley, Brian D
dc.contributor.cmtememberDurham, Dianne
dc.thesis.degreeDisciplineAnatomy & Cell Biology
dc.thesis.degreeLevelM.A.
kusw.oastatusna
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
kusw.bibid7643432
dc.rights.accessrightsopenAccessen_US


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