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    The interaction of ELKS with Ca2+ channel β subunits may contribute to active zone organization

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    Billings_ku_0099M_11840_DATA_1.pdf (11.00Mb)
    Issue Date
    2011-12-31
    Author
    Billings, Sara Elizabeth
    Publisher
    University of Kansas
    Format
    48 pages
    Type
    Thesis
    Degree Level
    M.A.
    Discipline
    Anatomy & Cell Biology
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    The cytoskeletal matrix of the active zone (CAZ) and synaptic voltage-dependent calcium channels (VDCCs) are both required for the organization and regulation of synaptic vesicle release. Here we report a novel interaction between the CAZ protein family ELKS and VDCC β subunits. We found that two isoforms of ELKS (ELKS2 and ELKS1b) and two isoforms of VDCC β subunits (β1b and β4) co-immunoprecipitated. Using fluorescent immunohistochemistry, we observed colocalization between ELKS1b and VDCC β4 at synapses in the molecular layer of the cerebellum, suggesting a synaptic role for the interaction of these two proteins. Analysis of a P/Q-type VDCC knockout mouse (Cacna1a-/-) revealed that the localization of the VDCC β4 subunit to the molecular layer was disrupted, although ELKS1b protein localization was not affected. The results demonstrate that two isoforms of the ELKS family and two VDCC β subunits interact and suggest that their interaction may play a role in the organization of the active zone.
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    http://hdl.handle.net/1808/9725
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    • KU Med Center Dissertations and Theses [464]
    • Theses [3828]

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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