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dc.contributor.authorManikwar, Prakash
dc.contributor.authorTejo, Bimo A.
dc.contributor.authorShinogle, Heather E.
dc.contributor.authorMoore, David S.
dc.contributor.authorZimmerman, Tahl
dc.contributor.authorBlanco, Francisco
dc.contributor.authorSiahaan, Teruna J.
dc.date.accessioned2011-06-15T14:36:34Z
dc.date.available2011-06-15T14:36:34Z
dc.date.issued2010-05-10
dc.identifier.citationManikwar P, Tejo BA, Shinogle H, Moore DS, Zimmerman T, Blanco F, Siahaan TJ. Utilization of I-domain of LFA-1 to Target Drug and Marker Molecules to Leukocytes. Theranostics 2011; 1:277-289.
dc.identifier.urihttp://hdl.handle.net/1808/7609
dc.description.abstractThe long-term objective of this project is to utilize the I-domain protein for the -subunit of LFA-1 to target drugs to lymphocytes by binding to ICAM receptors on the cell surface. The short-term goal is to provide proof-of-concept that I-domain conjugated to small molecules can still bind to and uptake by ICAM-1 on the surface of lymphocytes (i.e., Raji cells). To accomplish this goal, the I-domain protein was labeled with FITC at several lysine residues to produce the FITC-I-domain and CD spectroscopy showed that the FITC-I-domain has a secondary structure similar to that of the parent I-domain. The FITC-I-domain was taken up by Raji cells via receptor-mediated endocytosis and its uptake can be blocked by anti-I-domain mAb but not by its isotype control. Antibodies to ICAM-1 enhance the binding of I-domain to ICAM-1, suggesting it binds to ICAM-1 at different sites than the antibodies. The results in-dicate that fluorophore modification does not alter the binding and uptake properties of the I-domain protein. Thus, I-domain could be useful as a carrier of drug to target ICAM-1-expressing lymphocytes.
dc.language.isoen_US
dc.publisherTheranostics
dc.relation.isversionofhttp://www.thno.org/v01p0277.htm
dc.rights© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/ licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
dc.rights.urihttp://creativecommons.org/ licenses/by-nc-nd/3.0/
dc.subjectIcam-1
dc.subjectFitc-i-domain
dc.subjectBinding
dc.subjectEndocytosis
dc.subjectRaji cells
dc.titleUtilization of I-domain of LFA-1 to Target Drug and Marker Molecules to Leukocytes
dc.typeArticle
kusw.kuauthorManikwar, Prakash
kusw.kuauthorSiahaan, Teruna J.
kusw.kudepartmentPharmaceutical Chemistry
kusw.oastatusfullparticipation
dc.identifier.doi10.7150/thno/v01p0277
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/ licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
Except where otherwise noted, this item's license is described as: © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/ licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.