Receptor-mediated angiotensin II transcytosis by brain mcrovessel endothelial cells

Abstract

ROSE, J. M. AND K. L. AUDUS. Receptor-mediated angiotensin II transcytosis by brain microvessel endothelial cells. PEPTIDES __( ) _____, 199_. - Angiotensin II (Ang II) uptake and transport across monolayers of bovine brain microvessel endothelial cells (BMECs) was demonstrated. Ang II transport was linear up to 2 hours, saturable with a Km of 1.7 nM, and tended to be polarized with the apical-to-basolateral transport being greater. [3H]-Ang II transport was found to be inhibited by excess unlabeled Ang II, by the Ang II analog sarathrin, and by the endocytic inhibitor phenylarsine oxide. Ang II-(2-8) and -(3-8) were shown to significantly increase the transport of Ang II. These results demonstrate for the first time the receptor-mediated transcytosis of Ang II across brain microvessel endothelium.