KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    A Proteomic Study of Protein Tyrosine Nitration

    Thumbnail
    View/Open
    umi-ku-2708_1.pdf (3.306Mb)
    Issue Date
    2008-08-28
    Author
    Hong, Sung Jung
    Publisher
    University of Kansas
    Format
    147 pages
    Type
    Dissertation
    Degree Level
    PH.D.
    Discipline
    Pharmaceutical Chemistry
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
    Metadata
    Show full item record
    Abstract
    Protein post-translational modifications are of great interest due to their effects on protein structure and function. Our interest lays in the nitration of tyrosine residues by powerful oxidants such as peroxynitrite (ONOO-) forming 3-nitrotyrosine. The post-translational modification of tyrosine to 3-nitrotyrosine is a hallmark for protein oxidation. Traditional proteomics methods employed for the detection of nitrated peptides include SDS-PAGE, 2D-gel electrophoresis, Western blot, as well as immunoprecipitation. These are good and well accepted separation and detection methods, however they are only qualitative and don't tell us information about the specific sites of nitration. To overcome the drawbacks of these traditional proteomics methods, we have employed different methods that allowed us to perform all analytical steps in-solution. Also, we have explored the application of a novel 3-nitrotyrosine specific fluorescent, isotopic labeling technique developed in our labs for the sensitive detection and quantitation of 3-nitrotyrosine in different types of biological samples.
    URI
    http://hdl.handle.net/1808/4333
    Collections
    • Dissertations [4472]
    • Pharmaceutical Chemistry Dissertations and Theses [141]

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps